Study on the dielectrophoretic characteristics of malaria-infected red blood cells
Issued Date
2023-01-01
Resource Type
ISSN
01730835
eISSN
15222683
Scopus ID
2-s2.0-85172097797
Journal Title
Electrophoresis
Rights Holder(s)
SCOPUS
Bibliographic Citation
Electrophoresis (2023)
Suggested Citation
Panklang N., Vijitnukoonpradit K., Putaporntip C., Chotivanich K., Nakano M., Horprathum M., Techaumnat B. Study on the dielectrophoretic characteristics of malaria-infected red blood cells. Electrophoresis (2023). doi:10.1002/elps.202300088 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/90288
Title
Study on the dielectrophoretic characteristics of malaria-infected red blood cells
Other Contributor(s)
Abstract
Malaria is a tropical disease caused by parasites in the genus Plasmodium, which still presents 241 million cases and nearly 627,000 deaths recently. In this work, we used the dielectrophoresis (DEP) to characterize red blood cells in a microchannel. The purpose of this work is to determine the difference between the normal and the malaria-infected cells based on the DEP characteristics. The samples were infected cells and normal red blood cells, which were either prepared in culture or obtained from volunteers. Diamond-shaped and curved micropillars were used to create different degrees of DEP in the gap between them. The DEP crossover frequencies were observed with the diamond-shaped micropillars. The cell velocity under negative dielectrophoresis (nDEP) at a low frequency was examined with the curved micropillars. The measured lower crossover frequencies were remarkably different between the malaria-infected cells and the normal cells, whereas the higher crossover frequencies were similar among the samples. The velocity under nDEP was lower for the infected cells than the normal cells. The results imply that the malaria infection significantly decreases the capacitance but increases the conductance of the cell membrane, whereas a change in cytoplasmic conductivity may occur in a later stage of infection.