Function of Burkholderia pseudomallei RpoS and RpoN2 in bacterial invasion, intracellular survival, and multinucleated giant cell formation in mouse macrophage cell line
Issued Date
2024-12-01
Resource Type
ISSN
00036072
eISSN
15729699
Scopus ID
2-s2.0-85185667261
Journal Title
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume
117
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology Vol.117 No.1 (2024)
Suggested Citation
Diep D.T.H., Vong L.B., Tungpradabkul S. Function of Burkholderia pseudomallei RpoS and RpoN2 in bacterial invasion, intracellular survival, and multinucleated giant cell formation in mouse macrophage cell line. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology Vol.117 No.1 (2024). doi:10.1007/s10482-024-01944-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97412
Title
Function of Burkholderia pseudomallei RpoS and RpoN2 in bacterial invasion, intracellular survival, and multinucleated giant cell formation in mouse macrophage cell line
Author(s)
Corresponding Author(s)
Other Contributor(s)
Abstract
Melioidosis, a human infectious disease with a high mortality rate in many tropical countries, is caused by the pathogen Burkholderia pseudomallei (B. pseudomallei). The function of the B. pseudomallei sigma S (RpoS) transcription factor in survival during the stationary growth phase and conditions of oxidative stress is well documented. Besides the rpoS, bioinformatics analysis of B. pseudomallei genome showed the existence of two rpoN genes, named rpoN1 and rpoN2. In this study, by using the mouse macrophage cell line RAW264.7 as a model of infection, the involvement of B. pseudomallei RpoS and RpoN2 in the invasion, intracellular survival leading to the reduction in multinucleated giant cell (MNGC) formation of RAW264.7 cell line were illustrated. We have demonstrated that the MNGC formation of RAW264.7 cell was dependent on a certain number of intracellular bacteria (at least 5 × 104). In addition, the same MNGC formation (15%) observed in RAW264.7 cells infected with either B. pseudomallei wild type with multiplicity of infection (MOI) 2 or RpoN2 mutant (∆rpoN2) with MOI 10 or RpoS mutant (∆rpoS) with MOI 100. The role of B. pseudomallei RpoS and RpoN2 in the regulation of type III secretion system on bipB-bipC gene expression was also illustrated in this study.