Influence of fluoride on neuronal differentiation in dopaminergic differentiation SH-SY5Y cells
Issued Date
2022-01-01
Resource Type
eISSN
26300087
Scopus ID
2-s2.0-85148299231
Journal Title
Science, Engineering and Health Studies
Volume
16
Rights Holder(s)
SCOPUS
Bibliographic Citation
Science, Engineering and Health Studies Vol.16 (2022)
Suggested Citation
Limboonreung T., Chansiri S., Tsao S., Khwanraj K. Influence of fluoride on neuronal differentiation in dopaminergic differentiation SH-SY5Y cells. Science, Engineering and Health Studies Vol.16 (2022). doi:10.14456/sehs.2022.43 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/86627
Title
Influence of fluoride on neuronal differentiation in dopaminergic differentiation SH-SY5Y cells
Author(s)
Other Contributor(s)
Abstract
Fluoride is widely used in dentistry to prevent dental caries by increasing the fluoride content in saliva and aiding enamel remineralization. Excessive fluoride in the blood can cause adverse health effects such as fluorosis, which alter cerebral function. The influence of fluoride on dopaminergic neurons however, remains largely unclear. The present study examined the effect of sodium fluoride toxicity on dopaminergic neurons in retinoic acid-induced differentiation in SH-SY5Y cells. Cell viability was reduced by fluoride in both time- and concentration-dependent manners. Moreover, immunoblot analysis showed that fluoride decreased neuronal marker microtubule-associated protein-2 expression and levels of tyrosine hydroxylase, a rate-determining step enzyme in dopamine synthesis, even at a nonlethal dose. These results suggest that fluorosis may adversely affect dopaminergic neurons and may have harmful effects in individuals with degenerative dopaminergic neuron conditions, such as Parkinson's disease.