Induction of Osseointegration by Nacre in Pigs
Issued Date
2022-05-01
Resource Type
eISSN
14203049
Scopus ID
2-s2.0-85129252345
Pubmed ID
35566000
Journal Title
Molecules
Volume
27
Issue
9
Rights Holder(s)
SCOPUS
Bibliographic Citation
Molecules Vol.27 No.9 (2022)
Suggested Citation
Leelatian L., Chunhabundit P., Charoonrut P., Asvanund P. Induction of Osseointegration by Nacre in Pigs. Molecules Vol.27 No.9 (2022). doi:10.3390/molecules27092653 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86862
Title
Induction of Osseointegration by Nacre in Pigs
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Nacre is a biomaterial that has shown osteoinductive and osteoconductive properties in vitro and in vivo. These properties make nacre a material of interest for inducing bone regeneration. However, information is very limited regarding the introduction of nacre to dental implant surgery for promoting osteogenesis. This study investigated the potential of nacre powder for peri-implant bone regeneration in a porcine model. Ninety-six dental implants were placed into the tibia of twelve male domestic pigs. The dental implants were coated with nacre powder from the giant oyster before implantation. Implantations without nacre powder were used as control groups. Euthanization took place at 2, 4 and 6 weeks after implantation, after which we measured bone-to-implant contact (BIC) and bone volume density (BVD) of the implanted bone samples using micro-computed tomography (micro-CT), and examined the histology of the surrounding bone using histological sections stained with Stevenel’s blue and Alizarin red S. The micro-CT analyses showed that the BIC of dental implantations with nacre powder were significantly higher than those without nacre powder, by 7.60%. BVD of implantations with nacre powder were significantly higher than those without nacre powder, by 12.48% to 13.66% in cortical bone, and by 3.37% to 6.11% in spongy bone. Histological study revealed more peri-implant bone regeneration toward the surface of the dental implants after implantation with nacre powder. This was consistent with the micro-CT results. This study demonstrates the feasibility of using nacre to promote peri-implant bone regeneration in dental implantation.