Highly Active Carbon-Platinum-Based Nanozymes: Synthesis, Characterization, and Immunoassay Application
Issued Date
2024-01-01
Resource Type
eISSN
25740970
Scopus ID
2-s2.0-85211617884
Journal Title
ACS Applied Nano Materials
Rights Holder(s)
SCOPUS
Bibliographic Citation
ACS Applied Nano Materials (2024)
Suggested Citation
Kafil V., Sreenan B., Ra Shin S.H., Brennan A.A., Brett P.J., AuCoin D.P., Thallapally P.K., Tal-Gan Y., Zhu X. Highly Active Carbon-Platinum-Based Nanozymes: Synthesis, Characterization, and Immunoassay Application. ACS Applied Nano Materials (2024). doi:10.1021/acsanm.4c05224 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/102425
Title
Highly Active Carbon-Platinum-Based Nanozymes: Synthesis, Characterization, and Immunoassay Application
Corresponding Author(s)
Other Contributor(s)
Abstract
Nanozymes (nanomaterials with intrinsic enzyme-like characteristics) have gained much attention for diagnostics and therapy due to their excellent enzyme-mimicking capability, great stability in environments, and facile and low-cost production. However, developing nanozymes with a high catalytic constant, Kcat, has been challenging. Herein, we report a class of nanozyme-mimicking peroxidases, which are formed by depositing ultrasmall platinum nanoparticles (Pt NPs) 1-2 nm in size on the surface of hydrophilic nitrogen-doped carbon nanoparticles (CN NPs). These nanozymes defined as CN-Pt NPs show a high peroxidase-like activity with Kcat values of 1.27 M·mL/s·g for 3,3,5,5′-tetramethylbenzidine (TMB) and 1.97 M·mL/s·g for hydrogen peroxide (H2O2), respectively, which are at least one or two orders higher than many other reported carbon-noble metal-based nanozymes. Our developed CN-Pt NPs were further utilized in a colorimetric immunoassay as signal amplifiers for the biomarker detection of Burkholderia pseudomallei, a Gram-negative bacterial pathogen classified as a tier 1 select agent by the US CDC. The assay achieved lower limits of detection of 0.11 ng/mL in phosphate-buffered saline (PBS) and 0.16 ng/mL in human serum, when compared to many other assays in detecting the same biomarker.