Development of an immunochromatographic strip test for antigen detection of elephant endotheliotropic herpesvirus in Asian elephants (Elephas maximus)
Issued Date
2023-01-01
Resource Type
ISSN
01660934
eISSN
18790984
Scopus ID
2-s2.0-85139825671
Pubmed ID
36191664
Journal Title
Journal of Virological Methods
Volume
311
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Virological Methods Vol.311 (2023)
Suggested Citation
Guntawang T., Sittisak T., Srivorakul S., Photichai K., Aiumurai P., Thitaram C., Sthitmatee N., Hsu W.L., Sookrung N., Pringproa K. Development of an immunochromatographic strip test for antigen detection of elephant endotheliotropic herpesvirus in Asian elephants (Elephas maximus). Journal of Virological Methods Vol.311 (2023). doi:10.1016/j.jviromet.2022.114627 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81986
Title
Development of an immunochromatographic strip test for antigen detection of elephant endotheliotropic herpesvirus in Asian elephants (Elephas maximus)
Author's Affiliation
Other Contributor(s)
Abstract
Elephant endotheliotropic herpesvirus (EEHV) is the causative agent of EEHV-hemorrhagic disease (EEHV-HD) in elephants worldwide. This disease is highly virulent and a predominant cause of fatalities in young Asian elephants. Rapid diagnosis and aggressive therapies have been determined to be a key strategy in the successful treatment of this disease. Herein, we have developed the immunochromatographic strip test for EEHV detection. Accordingly, 31.2 kDa of partial EEHV DNA polymerase (DNApol) protein was expressed in Escherichia coli and used to generate rabbit polyclonal anti-EEHV DNApol antibodies. These were then used to develop an ICS test for EEHV antigen detection using the double-antibody sandwich colloidal gold method. Anti-EEHV DNApol antibodies conjugated with 40 nm colloidal gold solution were used as a detector, while rabbit anti-EEHV DNApol and goat anti-rabbit IgG antibodies immobilized on the nitrocellulose membrane were used as the test and control lines, respectively. The test had a detection limit of 1.25 × 105 viral genome copies (vgc)/mL of EEHV obtained from blood samples. Moreover, no specialized equipment or laboratory infrastructure was required in the administration of this test. This developed ICS test for EEHV antigen detection can be used in field application for the rapid detection of EEHV in resource-limited environments.