Iron uptake and metabolism in malaria parasite
Issued Date
2024
Copyright Date
1989
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xii, 122 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Biochemistry))--Mahidol University, 1989
Suggested Citation
Somdet Srichairatankool Iron uptake and metabolism in malaria parasite. Thesis (M.Sc. (Biochemistry))--Mahidol University, 1989. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/99953
Title
Iron uptake and metabolism in malaria parasite
Author(s)
Abstract
Iron uptake study of P.berghei-infected cells was investigated both in vitro and in vivo. The results showed no enhanced iron uptake by the infected cells in both system. Various sugar showed no effect on the iron uptake. Possible existence and the presence of specific iron chelator (Plasmodial siderophore) in the lysated of infected cells was evidenced, Iron mobilization studies showed that deproteinized infected lysate could chelate the iron from chrome azurol S-ferric complex and transferrin. More significantly, ferritin iron was strongly chelated by the infected lysates (both P.berghei and P.falciparum) The results suggested that ferritin iron might be serving as endogenous iron source for the parasite and that the lysates possibly contained parasite-derived iron chelator for iron aquisition. Organic solvent extraction showed that the iron chelator (Plasmodial siderophore) was extractable with benzyl alcohol but not with ethyl acetate. The results suggested the presence of hydroxamate type siderophore in the infected lysata. Methonol/water (1:1) was found to be suitable solvent for characterizing the plasmodial siderophores by paper chromatography. The R(,f) value of 0.44 for the infected lysate was found to be different from that of DFO (= 0.94) while the uninfected lysate remained at the origin. Iron competitive mobilization study showed desferrioxamine to be stronger chelator than that of the parasite. The results of this study suggested that intraerythrocytic parasite might be dependent on ferritin iron. The iron acquisition was probably by means of parasite siderophore. The siderophore was of hydroxamate type low molecular weight compound with R(,f) of 0.44 in paper chromatography with methanol/water (1:1) as solvent system.
Description
Biochemistry (Mahidol University 1989)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Biochemistry
Degree Grantor(s)
Mahidol University