Thiopurine S-methyltransferase (TPMT) activity cutoffs in the Thai population
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Issued Date
2025-01-01
Resource Type
ISSN
00045632
eISSN
17581001
Scopus ID
2-s2.0-105016504924
Pubmed ID
40919916
Journal Title
Annals of Clinical Biochemistry
Rights Holder(s)
SCOPUS
Bibliographic Citation
Annals of Clinical Biochemistry (2025)
Suggested Citation
Jinda P., Kitpoka P., Thienphopirak W., Chiawchan S., Prommas S., Sukprasong R., Rachanakul J., Wiwattanakul S., Suteerojntrakool O., Bongsebandhu-phubhakdi C., Tempark T., Hunthai S., Puangpetch A. Thiopurine S-methyltransferase (TPMT) activity cutoffs in the Thai population. Annals of Clinical Biochemistry (2025). doi:10.1177/00045632251381058 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/112279
Title
Thiopurine S-methyltransferase (TPMT) activity cutoffs in the Thai population
Corresponding Author(s)
Other Contributor(s)
Abstract
Background: Thiopurine S-methyltransferase (TPMT) is crucial for metabolizing thiopurine drugs. This study aimed to establish the cutoff values for TPMT activity in a cohort of healthy individuals. We defined normal TPMT activity ranges and identified clinically applicable thresholds to distinguish individuals with normal TPMT function from those with reduced or deficient activity. Methods: A total of 457 participants, including 207 children and 250 healthy adults without prior thiopurine drug exposure, were enrolled. TPMT activity was measured and common defective genetic variants (TPMT*3A, TPMT*3B, and TPMT*3C) were detected. To determine TPMT activity cutoff values and maximize sensitivity and specificity, receiver operating characteristic curve analysis was employed. Results: The cutoff values for TPMT activity in children were ≥52.9 nmol 6-MMP/g Hb/h for persons of the wild type and <52.9 nmol 6-MMP/g Hb/h for individuals who were heterozygous. In adults, the cutoff values were ≥44.6, 31.58–44.5, and <31.58 nmol 6-MMP/g Hb/h for wild-type, heterozygous, and compound heterozygous individuals, respectively. The sensitivity and specificity were 79.29% and 100% in children, whereas, in adults, they were 61.86% and 78.57%, 38.46% and 64.73%, and 100% and 95.98% in the wild-type, heterozygous, and compound heterozygous, respectively. Conclusions: Identifying TPMT activity cutoff values is crucial for managing patients receiving thiopurine therapy, especially in Thailand. This approach allows for personalized treatment plans and minimizes the risk of adverse drug reactions. Since TPMT activity cutoff values can differ by population and testing methods, it is important to establish specific cutoff values locally.