Evaluation of immunological effects of two DNA vaccines against Nocardia seriolae in hybrid snakehead
Issued Date
2025-06-01
Resource Type
ISSN
10504648
eISSN
10959947
Scopus ID
2-s2.0-86000742341
Journal Title
Fish and Shellfish Immunology
Volume
161
Rights Holder(s)
SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology Vol.161 (2025)
Suggested Citation
Wen Y., Chen G., Li Y., Ning D., Sirimanapong W., Xia L. Evaluation of immunological effects of two DNA vaccines against Nocardia seriolae in hybrid snakehead. Fish and Shellfish Immunology Vol.161 (2025). doi:10.1016/j.fsi.2025.110233 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/106795
Title
Evaluation of immunological effects of two DNA vaccines against Nocardia seriolae in hybrid snakehead
Corresponding Author(s)
Other Contributor(s)
Abstract
Although DNA vaccines hold significant potential, their practical application in aquaculture remains limited. In both mammals and teleost fish, B cells, which recognize antigens and produce antibodies, play an important role in immunity. In this study, B-cell epitopes capable of inducing fish immunity from IS701 family transposase (IS701) and molybdopterin-dependent oxidoreductase (Mol) proteins of Nocardia seriolae were screened. PcDNA-IS701 and pcDNA-Mol recombinant plasmids were constructed. The results showed that two proteins possessed multiple B-cell epitopes, and both pcDNA-IS701 and pcDNA-Mol induced innate immunity and specific antibody responses, along with increased mRNA expression levels of genes encoding humoral (MHCIIα and CD4) and cell-mediated (MHCIα and CD8α) immunity. In addition, both pcDNA-IS701 and pcDNA-Mol strengthened the protection against N. seriolae infection, with immune protection rates of 45.06 % for IS701 and 61.04 % for Mol, respectively. In conclusion, pcDNA-IS701 and pcDNA-Mol are candidate DNA vaccines for nocardiosis in fish.
