Comparison of effective dose with protection time for three botanicals by using multi-chamber-blood feeding system against Aedes aegypti
2
Issued Date
2024
Copyright Date
2020
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xiii, 77 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Tropical Medicine))--Mahidol University, 2019
Suggested Citation
Muhammad, Adnan, 1993- Comparison of effective dose with protection time for three botanicals by using multi-chamber-blood feeding system against Aedes aegypti. Thesis (M.Sc. (Tropical Medicine))--Mahidol University, 2019. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/92115
Title
Comparison of effective dose with protection time for three botanicals by using multi-chamber-blood feeding system against Aedes aegypti
Author(s)
Abstract
The testing of repellents has typically been performed on shaven animals, such as rabbits, dogs, guinea pigs, chickens, and sheep as alternative subjects to humans. However, these techniques often raise queries concerning the ethical treatment of animals and/or humans, as well as practical aspects that sometimes cause the results of repellent tests to be misconstrued. In this study, a test system has been developed and improved method for the evaluation of mosquito deterrence time. This multi chamber blood feeding method was designed to require fewer test mosquitoes than conventional cages and also to reduce the risk of contamination with repellents, to evaluate effective dose and deterrent time. DEET and three botanical oils were used for this test against Aedes aegypti. Absolute ethanol was used as a positive control. Method involved placing treated netting of different repellent concentrations over a cup containing test mosquitoes for 5 minutes. The time, in minutes, between placement of the net over the test cup until first mosquito bite, was recorded as deterrence time. Results showed that citronella had the highest deterrent time of 240 minutes at 50% concentration, while 100% lemon eucalyptus oil and lavender oil deterrence times were lower, at 150 and 120 min, respectively. The control group experienced a higher biting rate than the treatment groups. The effective dose (ED50) of the tested repellents, in ascending order, were DEET (0.50%), citronella (10%), lemon eucalyptus (33.11%), and lavender (33.88%), to deter 50% of the tested mosquito population. The data were analyzed using the Kruskal Wallis test to compare the groups for effective dose and plot (log dose and % inhibition). It is suggested that this method is effective for repellent testing and may also be used for testing mosquito susceptibility/resistance to insecticides. The method used circumvents the need to use humans or animals, and therefore overcomes historical ethical challenges.
Description
Tropical Medicine (Mahidol University 2020)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Tropical Medicine
Degree Discipline
Tropical Medicine
Degree Grantor(s)
Mahidol University