YAP and TAZ play a crucial role in human erythrocyte maturation and enucleation
Issued Date
2022-12-01
Resource Type
eISSN
17576512
Scopus ID
2-s2.0-85137607648
Pubmed ID
36076260
Journal Title
Stem Cell Research and Therapy
Volume
13
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Stem Cell Research and Therapy Vol.13 No.1 (2022)
Suggested Citation
Damkham N., Lorthongpanich C., Klaihmon P., Lueangamornnara U., Kheolamai P., Trakarnsanga K., Issaragrisil S. YAP and TAZ play a crucial role in human erythrocyte maturation and enucleation. Stem Cell Research and Therapy Vol.13 No.1 (2022). doi:10.1186/s13287-022-03166-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/85262
Title
YAP and TAZ play a crucial role in human erythrocyte maturation and enucleation
Author's Affiliation
Other Contributor(s)
Abstract
Background: Yes-associated protein (YAP) and WW domain-containing transcription regulator protein 1 (WWTR1, also known as TAZ) are two key transcription co-activators of the Hippo pathway. Both were originally characterized as organ size and cell proliferation regulators. Later studies demonstrated that the Hippo pathway may play a role in Drosophila and mammal hematopoiesis. However, the role of the Hippo pathway in human erythropoiesis has not yet been fully elucidated. Methods: The role of YAP and TAZ was studied in human erythropoiesis and hematopoietic stem cell (HSC) lineage determination by using mobilized peripheral blood (PB) and cord blood (CB)-derived HSC as a model. HSCs were isolated and cultured in an erythroid differentiation medium for erythroid differentiation and culture in methylcellulose assay for HSC lineage determination study. Results: YAP and TAZ were barely detectable in human HSCs, but became highly expressed in pro-erythroblasts and erythroblasts. Depletion or knockdown of YAP and/or TAZ did not affect the ability of HSC lineage specification to erythroid lineage in either methylcellulose assay or liquid culture. However, depletion of YAP and TAZ did impair erythroblast terminal differentiation to erythrocytes and their enucleation. Moreover, ectopic expression of YAP and TAZ in pro-erythroblasts did not exert an apparent effect on erythroid differentiation, expansion, or morphology. Conclusions: This study demonstrated that YAP/TAZ plays important role in erythroid maturation and enucleation but is dispensable for lineage determination of human HSCs.