Enhanced effectiveness of silver diamine fluoride application with light curing on natural dentin carious lesions: an in vitro study
Issued Date
2023-04-01
Resource Type
ISSN
16181247
eISSN
16181255
Scopus ID
2-s2.0-85140297333
Pubmed ID
36269519
Journal Title
Odontology
Volume
111
Issue
2
Start Page
439
End Page
450
Rights Holder(s)
SCOPUS
Bibliographic Citation
Odontology Vol.111 No.2 (2023) , 439-450
Suggested Citation
Karnowakul J., Punyanirun K., Jirakran K., Thanyasrisung P., Techatharatip O., Pornprasertsuk-Damrongsri S., Trairatvorakul C. Enhanced effectiveness of silver diamine fluoride application with light curing on natural dentin carious lesions: an in vitro study. Odontology Vol.111 No.2 (2023) , 439-450. 450. doi:10.1007/s10266-022-00755-z Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81811
Title
Enhanced effectiveness of silver diamine fluoride application with light curing on natural dentin carious lesions: an in vitro study
Other Contributor(s)
Abstract
This study aimed to compare the mean mineral density difference (mMDD) and surface morphology of 10- and 60-s silver diamine fluoride (SDF)-applied dentin carious lesions and to study the effect of an additional 20-s light curing (LC) on SDF-treated teeth. Forty primary molar blocks with natural dentin carious lesions were measured for baseline lesion depth and mineral density using Image-Pro Plus software. The samples were randomly distributed into 4 groups; 38% SDF applied for 1) 10-s (10SDF), 2) 60-s (60SDF), 3) 10-s + LC (10SDF + LC), 4) 60-s + LC (60SDF + LC) and an additional control group to assess the outcome of pH-cycling only. Then all the groups underwent a 7-d bacterial pH-cycling. The dentin carious lesions’ mMDD was determined by digital subtraction radiographic analysis. The surface morphology and elemental profile were assessed by scanning electron microscopy and energy-dispersive X-ray spectroscopy. The mMDD of the dentin lesions was analyzed using two-way ANOVA, generalized linear models analysis. Light curing was the only factor that affected the mMDD (p = 0.007). The mMDD in the 10SDF + LC and 60SDF + LC groups were significantly higher than those without light curing (p = 0.041 and 0.041, respectively). The 60SDF + LC group demonstrated a significantly higher mMDD than the 10SDF group (p = 0.010), while that in the 10SDF + LC group was similar to the 60SDF group (p = 1.00). Scanning electron microscopy revealed denser mineral content layers, which were likely silver and chloride, in the 10SDF + LC and 60SDF + LC groups than in the 10SDF and 60SDF groups, respectively. In conclusion, shortened application time with light curing enhanced SDF remineralization similarly to the conventional method.