IN SILICO IDENTIFICATION OF SECONDARY METABOLITES FROM AQUATIC STREPTOMYCES ISOLATE WITH ANTIYEAST AND ANTICANCER ACTIVITIES
1
Issued Date
2025-01-01
Resource Type
ISSN
0858849X
eISSN
25870009
Scopus ID
2-s2.0-105018588415
Journal Title
Suranaree Journal of Science and Technology
Volume
32
Issue
4
Rights Holder(s)
SCOPUS
Bibliographic Citation
Suranaree Journal of Science and Technology Vol.32 No.4 (2025)
Suggested Citation
Chalermsri P., Patthamasupchock W., Nunoi M., Naksutti W., Aroonsri A., Uawisetwathana U., Intra B., Chaiseeda K., Euanorasetr J. IN SILICO IDENTIFICATION OF SECONDARY METABOLITES FROM AQUATIC STREPTOMYCES ISOLATE WITH ANTIYEAST AND ANTICANCER ACTIVITIES. Suranaree Journal of Science and Technology Vol.32 No.4 (2025). doi:10.55766/sujst4524 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/112695
Title
IN SILICO IDENTIFICATION OF SECONDARY METABOLITES FROM AQUATIC STREPTOMYCES ISOLATE WITH ANTIYEAST AND ANTICANCER ACTIVITIES
Corresponding Author(s)
Other Contributor(s)
Abstract
This study explored the potential of bioactive metabolites from Streptomyces strain WHD3007-4. Isolated from a Nile tilapia pond, aquatic Streptomyces sp. WHD3007-4 was cultured in four media (International Streptomyces Project-2 Medium (ISP2), 301, Starch Casein Broth (SCB), and Bennet’s Broth (BN). Its secondary metabolites were extracted using ethyl acetate and acetone at a ratio of 15:2 (v/v). The cytotoxicity of all crude extracts on the human colorectal cancer cell line HCT-116 was assessed using the MTT assay. The percentage of viable HCT-116 cells was lowest with Streptomyces sp. WHD3007-4 in the SCB medium (36.20%). The antiyeast activity of the crude extract from Streptomyces sp. WHD3007-4 in SCB medium was assessed against Candida tropicalis (TISTR No. 5136) using the disk diffusion method. The crude extract effectively inhibited C. tropicalis growth at 2 mg. Known compounds in the crude extract from Streptomyces sp. WHD3007-4 in different media were identified using liquid chromatography-tandem mass spectrometry, with the data processed using MZmine 3 for molecular networking and spectral library searching on the Global Natural Products Social Molecular Networking (GNPS) web platform. Several candidate anticancer molecules were identified: kynurenic acid, brevianamide F, N-(2-hydroxyphenyl) acetamide, and decumbenone A. These results suggest that Streptomyces sp. WHD3007-4 is a potential source of antiyeast and anticancer agents.