Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion
Issued Date
2022-11-19
Resource Type
eISSN
19994915
Scopus ID
2-s2.0-85142626450
Pubmed ID
36423175
Journal Title
Viruses
Volume
14
Issue
11
Rights Holder(s)
SCOPUS
Bibliographic Citation
Viruses Vol.14 No.11 (2022)
Suggested Citation
Hitakarun A., Williamson M.K., Yimpring N., Sornjai W., Wikan N., Arthur C.J., Pompon J., Davidson A.D., Smith D.R. Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion. Viruses Vol.14 No.11 (2022). doi:10.3390/v14112566 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84873
Title
Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion
Other Contributor(s)
Abstract
A lipid bilayer produced from the host membrane makes up around 20% of the weight of the dengue virus (DENV) virion and is crucial for virus entry. Despite its significance, the virion's lipid composition is still poorly understood. In tandem with lipid profiles of the cells utilised to generate the virions, this work determined a partial lipid profile of DENV virions derived from two cell lines (C6/36 and LLC-MK2). The results showed distinctive profiles between the two cell types. In the mammalian LLC-MK2 cells, 30.8% (73/237 identified lipid species; 31 upregulated, 42 downregulated) of lipid species were altered in response to infection, whilst in insect C6/36 cells only 12.0% (25/208; 19 upregulated, 6 downregulated) of lipid species showed alterations in response to infection. For virions from LLC-MK2 cells, 14 lipids were detected specifically in virions with a further seven lipids being enriched (over mock controls). For virions from C6/36 cells, 43 lipids were detected that were not seen in mock preparations, with a further 16 being specifically enriched (over mock control). These results provide the first lipid description of DENV virions produced in mammalian and mosquito cells, as well as the lipid changes in the corresponding infected cells.