Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model
Issued Date
2023-12-01
Resource Type
eISSN
17466148
Scopus ID
2-s2.0-85145942946
Pubmed ID
36624444
Journal Title
BMC Veterinary Research
Volume
19
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
BMC Veterinary Research Vol.19 No.1 (2023)
Suggested Citation
Satitsri S., Akrimajirachoote N., Nunta K., Ruennarong N., Amnucksoradej O., Muanprasat C. Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model. BMC Veterinary Research Vol.19 No.1 (2023). doi:10.1186/s12917-022-03536-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81360
Title
Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model
Author's Affiliation
Other Contributor(s)
Abstract
Post-weaning diarrhea in piglets is a major problem, resulting in a significant loss in pig production. This study aimed to investigate the effects of piperine, an alkaloid abundantly found in black peppers, on biological activities related to the pathogenesis of post-weaning diarrhea using a porcine duodenal enteroid model, a newly established intestinal stem cell-derived in vitro model recapitulating physiology of porcine small intestinal epithelia. Porcine duodenal enteroid models were treated with disease-relevant pathological inducers with or without piperine (8 μg/mL and/or 20 μg/mL) before measurements of oxidative stress, mRNA, and protein expression of proinflammatory cytokines, nuclear factor-kappa B (NF-κB) nuclear translocation, barrier leakage, and fluid secretion. We found that piperine (20 μg/mL) inhibited H2O2-induced oxidative stress, TNF-α-induced mRNA, and protein expression of proinflammatory cytokines without affecting NF-κB nuclear translocation, and prevented TNF-α-induced barrier leakage in porcine duodenal enteroid monolayers. Importantly, piperine inhibited fluid secretion induced by both forskolin and heat-stable toxins (STa) in a three-dimensional model of porcine duodenal enteroids. Collectively, piperine possesses both anti-inflammatory and anti-secretory effects in porcine enteroid models. Further research and development of piperine may provide novel interventions for the treatment of post-weaning porcine diarrhea.