Comparative analysis of markers for H<inf>2</inf>O<inf>2</inf>-induced senescence in renal tubular cells
Issued Date
2023-01-01
Resource Type
ISSN
13826689
eISSN
18727077
Scopus ID
2-s2.0-85144453853
Pubmed ID
36528215
Journal Title
Environmental Toxicology and Pharmacology
Volume
97
Rights Holder(s)
SCOPUS
Bibliographic Citation
Environmental Toxicology and Pharmacology Vol.97 (2023)
Suggested Citation
Rattananinsruang P., Noonin C., Yoodee S., Thongboonkerd V. Comparative analysis of markers for H<inf>2</inf>O<inf>2</inf>-induced senescence in renal tubular cells. Environmental Toxicology and Pharmacology Vol.97 (2023). doi:10.1016/j.etap.2022.104039 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81893
Title
Comparative analysis of markers for H<inf>2</inf>O<inf>2</inf>-induced senescence in renal tubular cells
Author's Affiliation
Other Contributor(s)
Abstract
To address what marker(s) is/are most suitable for determining renal cell senescence, cell area, granularity, cycle shift/arrest, SA-β-Gal, SIRT1 and p16 were evaluated after inducing senescence in HK-2 cells with 0.2–0.8 mM H2O2. Only cell area and granularity concentration-dependently increased at all time-points, whereas SA-β-Gal, SIRT1 and p16 showed significant coefficient of determination (R2) at two time-points. Cell granularity had significant correlation coefficient (R) with other six, whereas SA-β-Gal had significant R with five, and cell area, SIRT1 and p16 had significant R with four others. Comparing to SA-β-Gal, other markers had significantly lower fold-changes only at 72-h with 0.8 mM H2O2, whereas p16 provided greater fold-changes at 48-h with 0.4 and 0.8 mM H2O2. Therefore, cell area, granularity, SA-β-Gal and p16 may serve as the most suitable markers for determining H2O2-induced senescence in HK-2 renal cells, whereas other markers can be also used but with inferior quantitative precision.
