Impairment of Invasion and Maturation and Decreased Selectivity of Plasmodium falciparum in G6PD Viangchan and Mahidol Variants
Issued Date
2022-04-01
Resource Type
ISSN
00221899
eISSN
15376613
Scopus ID
2-s2.0-85128161092
Pubmed ID
34558618
Journal Title
Journal of Infectious Diseases
Volume
225
Issue
7
Start Page
1238
End Page
1247
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Infectious Diseases Vol.225 No.7 (2022) , 1238-1247
Suggested Citation
Palasuwan D., Palasuwan A., Boonpeng K., Ketprasit N., Imwong M., Kulkeaw K. Impairment of Invasion and Maturation and Decreased Selectivity of Plasmodium falciparum in G6PD Viangchan and Mahidol Variants. Journal of Infectious Diseases Vol.225 No.7 (2022) , 1238-1247. 1247. doi:10.1093/infdis/jiab484 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/85985
Title
Impairment of Invasion and Maturation and Decreased Selectivity of Plasmodium falciparum in G6PD Viangchan and Mahidol Variants
Other Contributor(s)
Abstract
Background: Protection against Plasmodium falciparum is observed in a population deficient in glucose-6-phosphate dehydrogenase (G6PD), particularly in African and Mediterranean regions. However, such protection remains unknown among G6PD-deficient individuals in Southeast Asia. Methods: In this study, we assessed the invasion and maturation of P falciparum K1 in a culture of erythrocytes isolated from Thai subjects carrying Viangchan (871G>A) and Mahidol (487G>A). Results: We found that the parasites lost their ability to invade hemizygous and homozygous G6PD-deficient erythrocytes of Viangchan and Mahidol variants in the second and third cycles of intraerythrocytic development. It is interesting to note that P falciparum parasites selectively grew in erythrocytes from hemi-and homozygous genotypes with normal G6PD activity. Moreover, externalization of phosphatidylserine upon P falciparum infection was significantly increased only in Viangchan hemizygous variant cells. Conclusions: This study is the first to show that blockage of invasion in long-Term culture and potentially enhanced removal of parasitized erythrocytes were observed for the first time in erythrocytes from Viangchan and Mahidol G6PD-deficient individuals.