Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand
5
Issued Date
2025-01-21
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85216516792
Pubmed ID
39838039
Journal Title
Scientific reports
Volume
15
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific reports Vol.15 No.1 (2025) , 2620
Suggested Citation
Tapaopong P., da Silva G., Holzschuh A., Rungsarityotin W., Suansomjit C., Pumchuea K., Manopwisedjaroen K., Khamsiriwatchara A., Khuntong P., Cui L., Koepfli C., Sattabongkot J., Nguitragool W. Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand. Scientific reports Vol.15 No.1 (2025) , 2620. doi:10.1038/s41598-025-86578-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/104252
Title
Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand
Corresponding Author(s)
Other Contributor(s)
Abstract
The evolution of genetic diversity and population structure of Plasmodium vivax as malaria elimination approaches remains unclear. This study analyzed the genetic variation and molecular epidemiology of P. vivax from Yala Province in southern Thailand, an area in the pre-elimination phase. Seventy P. vivax isolates, collected between 2017 and 2020, were genotyped for domain II of pvdbp and the 42-kDa region of pvmsp1 using amplicon deep sequencing. Data from Yala province were compared to published data from Tak province, where transmission was higher. Key analyses included nucleotide diversity (π), haplotype diversity (Hd), natural selection, recombination rates, and complexity of infection (COI). Genetic diversity in Yala was relatively low (π = 0.008dbp and 0.014msp1; Hd = 0.774dbp and 0.407msp1) compared to Tak (π = 0.012dbp and 0.027msp1; Hd = 0.849dbp and 0.962msp1). In Yala, polyclonal infections were found in 53.7% of pvdbpII and 47.8% of pvmsp142 isolates, with average COI of 1.6 and 1.7. Both genes were under balancing selection. Distinct genetic differences were found between Yala and Tak in pvmsp142, providing a local genotypic profile useful for tracing parasite origins.