Integration of Group A Streptococcus Rapid Tests with the Open Fluidic CandyCollect Device
Issued Date
2025-01-01
Resource Type
ISSN
00032700
eISSN
15206882
Scopus ID
2-s2.0-105001721475
Journal Title
Analytical Chemistry
Rights Holder(s)
SCOPUS
Bibliographic Citation
Analytical Chemistry (2025)
Suggested Citation
Sanchez J.C., Robertson I.H., Shinkawa V.A.M., Su X., Tu W.C., Robinson T.R., Chang M.M., Blom A., Alfaro E., Hatchett D.B., Olanrewaju A.O., Wald E.R., DeMuri G.P., Berthier E., Thongpang S., Theberge A.B. Integration of Group A Streptococcus Rapid Tests with the Open Fluidic CandyCollect Device. Analytical Chemistry (2025). doi:10.1021/acs.analchem.4c05063 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/109547
Title
Integration of Group A Streptococcus Rapid Tests with the Open Fluidic CandyCollect Device
Corresponding Author(s)
Other Contributor(s)
Abstract
The CandyCollect is a lollipop-inspired open-fluidic oral sampling device designed to provide a comfortable user sampling experience. We demonstrate that the CandyCollect device can be coupled to a rapid antigen detection test (RADT) kit designed for Group A Streptococcus (GAS). Through in vitro experiments with pooled saliva spiked with Streptococcus pyogenes, we tested various reagents and elution volumes to optimize the RADT readout from CandyCollect device samples. The resulting optimized protocol uses the kit-provided reagents and lateral flow assay (LFA) while replacing the kit’s pharyngeal swab with the CandyCollect device, reducing the elution solution volume, and substituting the tube used for elution to accommodate the CandyCollect device. Positive test results were detected by eye with bacterial concentrations as low as the manufacturer’s “minimal detection limit” of 1.5 × 105 CFU/mL. LFA strips were also scanned and semiquantified with image analysis software to determine the signal-to-baseline ratio (SBR) and categorize positive test results without human bias. We tested our optimized protocol for integrating CandyCollect and RADT using CandyCollect clinical samples from pediatric patients (n = 6) who were previously diagnosed with GAS pharyngitis via pharyngeal swabs tested with RADT as part of their clinical care. The LFA results of these CandyCollect devices and interspersed negative controls were determined by independent observers with positive results obtained in four of the six participants on at least one LFA replicate. Taken together, our results show that CandyCollect devices from children with GAS pharyngitis can be tested by using LFA rapid tests.