Comparative and integrative single cell analysis reveals new insights into the transcriptional immaturity of stem cell-derived β cells
Issued Date
2024-12-01
Resource Type
eISSN
14712164
Scopus ID
2-s2.0-85182990882
Pubmed ID
38267908
Journal Title
BMC Genomics
Volume
25
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
BMC Genomics Vol.25 No.1 (2024)
Suggested Citation
Schmidt M.D., Ishahak M., Augsornworawat P., Millman J.R. Comparative and integrative single cell analysis reveals new insights into the transcriptional immaturity of stem cell-derived β cells. BMC Genomics Vol.25 No.1 (2024). doi:10.1186/s12864-024-10013-x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/95617
Title
Comparative and integrative single cell analysis reveals new insights into the transcriptional immaturity of stem cell-derived β cells
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Corresponding Author(s)
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Abstract
Diabetes cell replacement therapy has the potential to be transformed by human pluripotent stem cell-derived β cells (SC-β cells). However, the precise identity of SC-β cells in relationship to primary fetal and adult β-cells remains unclear. Here, we used single-cell sequencing datasets to characterize the transcriptional identity of islets from in vitro differentiation, fetal islets, and adult islets. Our analysis revealed that SC-β cells share a core β-cell transcriptional identity with human adult and fetal β-cells, however SC-β cells possess a unique transcriptional profile characterized by the persistent expression and activation of progenitor and neural-biased gene networks. These networks are present in SC-β cells, irrespective of the derivation protocol used. Notably, fetal β-cells also exhibit this neural signature at the transcriptional level. Our findings offer insights into the transcriptional identity of SC-β cells and underscore the need for further investigation of the role of neural transcriptional networks in their development.