Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis
1
Issued Date
2025-12-01
Resource Type
eISSN
1478811X
Scopus ID
2-s2.0-105010529564
Journal Title
Cell Communication and Signaling
Volume
23
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Cell Communication and Signaling Vol.23 No.1 (2025)
Suggested Citation
Lertprapai C., Peerapen P., Thongboonkerd V. Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis. Cell Communication and Signaling Vol.23 No.1 (2025). doi:10.1186/s12964-025-02345-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/111293
Title
Effects of calcium oxalate crystals on neutrophil cellular proteome and functions: implications for nephrolithiasis
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Abstract
Background: The majority of stone formers (87.5–95.9%) exhibit mild to moderate interstitial inflammation surrounding the stone. Neutrophils and neutrophil-derived genes/proteins have been found in renal papillae, stone matrix and urine of calcium oxalate monohydrate (COM) stone formers. However, neutrophil-crystal interactions, especially responses of neutrophils to COM crystals, remained unknown. Methods: This study addressed the effects of COM crystals on neutrophil cellular proteome and functions, including phagocytosis, activation/degranulation, neutrophil extracellular traps (NETs) formation and reactive oxygen species (ROS) production. Results: Label-free quantitative (LFQ) proteomics using nanoLC-ESI-Qq-TOF MS/MS with highly stringent criteria revealed that COM caused altered levels of 22 neutrophil proteins involved mainly in immune responses. Investigating neutrophil innate immune functions using flow cytometry, immunofluorescence/fluorescence imaging, ELISA and dichlorodihydrofluorescein diacetate (DCFH-DA) assay revealed that COM enhanced neutrophil phagocytic activity, NETs formation, activation/degranulation and ROS production. Moreover, secretome (a set of secretory products) from COM-treated neutrophils induced the recruitment of macrophages to phagocytose the COM-treated neutrophils. Conclusions: These findings illustrate the expression and functional responses of neutrophils to COM crystals and implicate the important roles that neutrophils play in nephrolithiasis.