A first attempt at determining the antibody-specific pattern of Platynosomum fastosum crude antigen and identification of immunoreactive proteins for immunodiagnosis of feline platynosomiasis
Issued Date
2022-08-01
Resource Type
ISSN
09728988
eISSN
22310916
Scopus ID
2-s2.0-85135902292
Journal Title
Veterinary World
Volume
15
Issue
8
Start Page
2029
End Page
2038
Rights Holder(s)
SCOPUS
Bibliographic Citation
Veterinary World Vol.15 No.8 (2022) , 2029-2038
Suggested Citation
Soe B.K., Adisakwattana P., Reamtong O., Anuracpreeda P., Sukhumavasi W. A first attempt at determining the antibody-specific pattern of Platynosomum fastosum crude antigen and identification of immunoreactive proteins for immunodiagnosis of feline platynosomiasis. Veterinary World Vol.15 No.8 (2022) , 2029-2038. 2038. doi:10.14202/vetworld.2022.2029-2038 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87077
Title
A first attempt at determining the antibody-specific pattern of Platynosomum fastosum crude antigen and identification of immunoreactive proteins for immunodiagnosis of feline platynosomiasis
Other Contributor(s)
Abstract
Background and Aim: Feline platynosomiasis, also known as lizard poisoning, is a feline hepatic disease caused by the parasitic trematode Platynosomum fastosum. Since this helminth resides in biliary ducts and gallbladder, the heavy infection can lead to failure of the hepatobiliary system and can be associated with cholangiocarcinoma. The primary diagnostic tool currently used is conventional fecal microscopy. However, low sensitivity of detection could occur in the case of light infection or biliary obstruction. This study aimed to determine the antibody-specific pattern of P. fastosum crude antigen and to identify immunoreactive proteins to develop the immunodiagnostic techniques. Materials and Methods: We investigated potential antigens specific to P. fastosum infection using western blotting. Forty-six samples of cat serum, including 16 P. fastosum-infected sera, eight healthy control sera, and 22 sera infected with other endoparasites were used. The sensitivity, specificity, positive predictive value, and negative predictive value of each band were calculated. Immunoreactive bands with high diagnostic values were further analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify the protein components. Results: Using immunoblotting, three proteins of 72 kDa, 53 kDa, and 13 kDa were found to be immunogenic. LC-MS/MS identified these proteins as a 70 kDa heat shock protein, a hypothetical protein (CRM22_002083) (adenosine triphosphate synthase subunit beta), and histone H2B, respectively. Conclusion: This study is the first to reveal three proteins that could be candidates for developing diagnostic tools for feline platynosomiasis.