Gold nanoparticles coated with collagen-I and their wound healing activity in human skin fibroblast cells
Issued Date
2024-07-15
Resource Type
ISSN
24058440
Scopus ID
2-s2.0-85196740132
Journal Title
Heliyon
Volume
10
Issue
13
Rights Holder(s)
SCOPUS
Bibliographic Citation
Heliyon Vol.10 No.13 (2024)
Suggested Citation
Poomrattanangoon S., Pissuwan D. Gold nanoparticles coated with collagen-I and their wound healing activity in human skin fibroblast cells. Heliyon Vol.10 No.13 (2024). doi:10.1016/j.heliyon.2024.e33302 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/99233
Title
Gold nanoparticles coated with collagen-I and their wound healing activity in human skin fibroblast cells
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Abstract
The slow wound healing process has become a major health problem. Gold nanoparticles (AuNPs) have been used in various biomedical applications because of their unique properties. Type I collagen (Collagen-I) is a protein and be the most abundant type of collagen. This type of collagen can help the surrounding structure to maintain its rigidity. In this study, we stabilized the surface of AuNPs using Collagen-I (Collagen-I@AuNPs) and investigated the effect of Collagen-I@AuNPs on wound healing. The evaluation of inflammatory cytokine secretion, which were interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α), was performed. We found that Collagen-I@AuNPs reduced the levels of IL-6 and TNF-α in scratched human skin fibroblast (HSF) cells. Furthermore, Collagen-I@AuNPs induced the expression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), which are key growth factors involved in wound healing. This results in enhanced wound closure. In addition, Collagen-I@AuNPs were not toxic to HSF cells and facilitated the cellular uptake of particles inside HSF cells. Therefore, Collagen-I@AuNPs is a promising candidate for wound healing enhancement.