Preservation of blood fed Aedes albopictus from field to laboratory and its incidence on host species identification
Issued Date
2013
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Raweewan Srisawat, รวีวรรณ ศรีสวัสดิ์, Sungsit Sungvornyothin, สังสิทธิ์ สังวรโยธิน, Maxime Jacquet, Narumon Komalamisra, นฤมล โกมลมิศร์, Chamnarn Apiwathnasorn, ชำนาญ อภิวัฒนศร, Jean-Pierre Dujardin, Sebastien Boyer (2013). Preservation of blood fed Aedes albopictus from field to laboratory and its incidence on host species identification. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63338
Title
Preservation of blood fed Aedes albopictus from field to laboratory and its incidence on host species identification
Other Contributor(s)
Abstract
Aedes albopictus is the major vector of Chikungunya virus in Thailand and Southeast Asian countries.
Blood meal characterizations are necessary to determine its natural feeding behaviour and on which
host the females are feeding. This research determined the best laboratory preservation method for
blood source detection of field mosquitoes. Female Ae. albopictus were fed with human blood and harvested
at 0, 24, 48, 72 and 96 hours afterward. Then, females were either: (1) preserved at – 20°C, (2) air-dried, (3)
dried by lantern, (4) immersed in absolute ethanol, (5) placed in Carnoy’s solution or (6) their abdomen were
crushed on filter paper. After 24 hours, every sample was stocked at -20°C. The blood meal was detectable up
to 24 hours post-feeding with ELISA tests. The optimal preservation method for ELISA test was ethanol. All the
preservation methods were positive with PCR techniques up to 48 hours, except the Carnoy’s solution method
which was less efficient. We propose the use of the filter paper or ethanol as most convenient and efficient
methods to preserve field mosquitoes until laboratory analysis either by ELISA or PCR.
Description
Joint International Tropical Medicine Meeting 2013: "Towards global health: an Asian paradigm of Tropical Medicine" 11-13 December 2013 Centara Grand & Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.147.