Enhancement of intestinal tight junction assembly by Coffea arabica pulp aqueous extract: mechanism of action and role of SIRT-1
Issued Date
2025-01-01
Resource Type
ISSN
26624052
eISSN
26624060
Scopus ID
2-s2.0-85216613565
Journal Title
Advances in Traditional Medicine
Rights Holder(s)
SCOPUS
Bibliographic Citation
Advances in Traditional Medicine (2025)
Suggested Citation
Sukmak P., Thongnak L., Wachiradejkul W., Inchai J., Chindaduangratn N., Kitti-udom N., Limwattananon T., Choksukchalalai N., Satianrapapong W., Hankan S., Amornlerdpison D., Ontawong A., Akrimajirachoote N., Aonbangkhen C., Muanprasat C., Vaddhanaphuti C.S., Pongkorpsakol P. Enhancement of intestinal tight junction assembly by Coffea arabica pulp aqueous extract: mechanism of action and role of SIRT-1. Advances in Traditional Medicine (2025). doi:10.1007/s13596-025-00817-x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/104260
Title
Enhancement of intestinal tight junction assembly by Coffea arabica pulp aqueous extract: mechanism of action and role of SIRT-1
Author's Affiliation
Center of Excellence on Petrochemical and Materials Technology
University of Phayao
Faculty of Medicine, Chiang Mai University
Chulalongkorn University
Chulabhorn Royal Academy
Kasetsart University
Maejo University
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Laboratory of Epithelial Tight Junction Pathophysiology
University of Phayao
Faculty of Medicine, Chiang Mai University
Chulalongkorn University
Chulabhorn Royal Academy
Kasetsart University
Maejo University
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Laboratory of Epithelial Tight Junction Pathophysiology
Corresponding Author(s)
Other Contributor(s)
Abstract
Intestinal tight junction disruption is considered as one of key pathogenic factors of several diseases including inflammatory bowel diseases. At present, there is no FDA-approved drug targeting intestinal tight junction recovery. Coffea arabica pulp is an agricultural waste but its aqueous extract contains a number of polyphenol-rich, bioactive compounds. The main aim of this study was to elucidate the pharmacological effects of Coffea arabica pulp aqueous extract (CPE) on intestinal tight junction re-assembly. Transepithelial electrical resistance (TER) measurement indicated that CPE significantly enhanced TER across the intestinal epithelial-like T84 cell monolayers in a time- and dose-dependent manner with a maximal effect being observed at 1,000 µg/ml. MTT assay and nuclear staining indicated that CPE had no cytotoxic effect on T84 cells. Fluorescein isothiocyanate (FITC)-dextran permeability assay demonstrated that CPE suppressed intestinal tight junction-dependent leak pathway permeability. In addition, the effect of CPE on enhancing intestinal tight junction assembly was not affected by inhibitors of calcium/calmodulin-dependent protein kinase kinase β (CaMKKβ), AMP-activated protein kinase (AMPK), and extracellular signal-regulated kinase (ERK). Surprisingly, sirtuin-1 (SIRT-1) inhibitors abrogated CPE-induced tight junction assembly in T84 cell monolayers. Furthermore, immunostaining indicated that CPE enhanced re-distribution of occludin and zonula occludens-1 (ZO-1) to cell junction region via SIRT-1-dependent mechanism. Collectively, CPE may be useful in the treatment of diseases related to intestinal tight junction disruption.