14-Deoxy-11,12-didehydroandrographolide Promotes Ex Vivo Expansion of Umbilical Cord Blood Stem Cells through Stemness-Related Gene Regulation
Issued Date
2026-04-21
Resource Type
eISSN
24701343
Scopus ID
2-s2.0-105036396021
Journal Title
ACS Omega
Volume
11
Issue
15
Start Page
22571
End Page
22580
Rights Holder(s)
SCOPUS
Bibliographic Citation
ACS Omega Vol.11 No.15 (2026) , 22571-22580
Suggested Citation
Ruknarong L., Sutjarit N., Boonmuen N., Iaocharoen P., Duangsong T., Pabuprapap W., Suksamrarn A., Tantikanlayaporn D. 14-Deoxy-11,12-didehydroandrographolide Promotes Ex Vivo Expansion of Umbilical Cord Blood Stem Cells through Stemness-Related Gene Regulation. ACS Omega Vol.11 No.15 (2026) , 22571-22580. 22580. doi:10.1021/acsomega.5c08975 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116415
Title
14-Deoxy-11,12-didehydroandrographolide Promotes Ex Vivo Expansion of Umbilical Cord Blood Stem Cells through Stemness-Related Gene Regulation
Corresponding Author(s)
Other Contributor(s)
Abstract
Umbilical cord blood (UCB) is a valuable alternative source of hematopoietic stem and progenitor cells (HSPCs) for allogeneic transplantation. However, the limited number of HSPCs that can be obtained from a single UCB unit remains a significant clinical challenge. Therefore, strategies to enhance the ex vivo expansion of functional HSPCs are of considerable interest. A key requirement for improving the success of HSC-based therapies, including transplantation and gene editing, is the ability to expand or preserve functional human HSCs during ex vivo culture. In this study, we demonstrate that 14-deoxy-11,12-didehydroandrographolide (14-DDA), a diterpenoid isolated from Andrographis paniculata, significantly promotes the ex vivo expansion of UCB-derived CD34<sup>+</sup> cells and enriches for primitive HSPC subsets (CD34<sup>+</sup>CD38<sup>–</sup>CD90<sup>+</sup>). Functional assays reveal that 14-DDA enhances colony-forming unit output, which preserves multilineage differentiation potential. Furthermore, 14-DDA activates the Wnt/β-catenin signaling pathway, indicating a potential mechanism for supporting stem cell self-renewal. Gene expression profiling reveals upregulation of stemness- and proliferation-associated genes, while suppression of genes is related to differentiation, stress responses, and oncogenesis. These findings suggest that 14-DDA promotes the expansion of functional HSPCs while maintaining their primitive phenotype and reducing oncogenic risk, highlighting its potential as a natural small molecule for enhancing hematopoietic stem cell-based therapies.