Fabrication of synbiotic carbohydrate polymer-based microcapsules: Effect of prebiotics on probiotic viability during freeze-drying, gastrointestinal transit and storage
Issued Date
2025-07-01
Resource Type
ISSN
01448617
Scopus ID
2-s2.0-105002332399
Journal Title
Carbohydrate Polymers
Volume
359
Rights Holder(s)
SCOPUS
Bibliographic Citation
Carbohydrate Polymers Vol.359 (2025)
Suggested Citation
Thinkohkaew K., Jonjaroen V., Niamsiri N., McClements D.J., Panya A., Suppavorasatit I., Potiyaraj P. Fabrication of synbiotic carbohydrate polymer-based microcapsules: Effect of prebiotics on probiotic viability during freeze-drying, gastrointestinal transit and storage. Carbohydrate Polymers Vol.359 (2025). doi:10.1016/j.carbpol.2025.123582 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/109584
Title
Fabrication of synbiotic carbohydrate polymer-based microcapsules: Effect of prebiotics on probiotic viability during freeze-drying, gastrointestinal transit and storage
Corresponding Author(s)
Other Contributor(s)
Abstract
The stability and efficacy of probiotics can be significantly enhanced through advanced encapsulation technologies. This study aimed to investigate the impact of co-encapsulating probiotic with prebiotics in chitosan coated–alginate/gellan gum microcapsules on their viability during freeze-drying, gastrointestinal transit, and storage. Five prebiotics were added to microcapsules to assess their impact on probiotic viability during freeze-drying, including inulin, fructo-oligosaccharide (FOS), galacto-oligosaccharide (GOS), xylo-oligosaccharide (XOS), and resistant dextrin (RD). Notably, FOS–integrated microcapsules at concentration of 4 wt% exhibited the highest stability, achieving 83.36 % probiotic survival rate post-freeze-drying, a 28 % increase over those without FOS. Physicochemical analysis revealed that 4 % FOS–integrated microcapsules exhibited a particle size of 523.53 μm, a pore size of 17.2 μm, a moisture content of 3.57 %, and aw of 0.246, contributing to enhanced probiotic retention. The addition of FOS maintained probiotic viability at ∼6.4 log CFU/g after gastrointestinal digestion, following Korsmeyer–Peppas release kinetics (R2 = 0.9035). Additionally, these microcapsules sustained probiotic levels (> 6 log CFU/g) for 90 days at 4 °C and 7 days at 25 °C, highlighting their long-term storage potential. These findings provide valuable insights into designing resilient synbiotic-microcapsules for functional foods, supplements, and therapeutic applications, ensuring enhanced probiotic stability and efficacy under real-world conditions.