Soil Microbiomes and their Arsenic Functional Genes in Chronically High-Arsenic Contaminated Soils
Issued Date
2024-03-01
Resource Type
ISSN
00074861
eISSN
14320800
Scopus ID
2-s2.0-85187237312
Journal Title
Bulletin of Environmental Contamination and Toxicology
Volume
112
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
Bulletin of Environmental Contamination and Toxicology Vol.112 No.3 (2024)
Suggested Citation
Sonthiphand P., Rueangmongkolrat N., Uthaipaisanwong P., Kusonmano K., Mhuantong W., Termsaithong T., Limthamprasert C., Chotpantarat S., Luepromchai E. Soil Microbiomes and their Arsenic Functional Genes in Chronically High-Arsenic Contaminated Soils. Bulletin of Environmental Contamination and Toxicology Vol.112 No.3 (2024). doi:10.1007/s00128-024-03866-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97695
Title
Soil Microbiomes and their Arsenic Functional Genes in Chronically High-Arsenic Contaminated Soils
Corresponding Author(s)
Other Contributor(s)
Abstract
Microbial arsenic transformations play essential roles in controlling pollution and ameliorating risk. This study combined high-throughput sequencing and PCR-based approaches targeting both the 16 S rRNA and arsenic functional genes to investigate the temporal and spatial dynamics of the soil microbiomes impacted by high arsenic contamination (9.13 to 911.88 mg/kg) and to investigate the diversity and abundance of arsenic functional genes in soils influenced by an arsenic gradient. The results showed that the soil microbiomes were relatively consistent and mainly composed of Actinobacteria (uncultured Gaiellales and an unknown_67 − 14 bacterium), Proteobacteria, Firmicutes (particularly, Bacillus), Chloroflexi, and Acidobacteria (unknown_Subgroup_6). Although a range of arsenic functional genes (e.g., arsM, arsC, arrA, and aioA) were identified by shotgun metagenomics, only the arsM gene was detected by the PCR-based method. The relative abundance of the arsM gene accounted for 0.20%–1.57% of the total microbial abundance. Combining all analyses, arsenic methylation mediated by the arsM gene was proposed to be a key process involved in the arsenic biogeochemical cycle and mitigation of arsenic toxicity. This study advances our knowledge about arsenic mechanisms over the long-term in highly contaminated soils.