Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress
Issued Date
2022-03-01
Resource Type
ISSN
22211691
Scopus ID
2-s2.0-85127063875
Journal Title
Asian Pacific Journal of Tropical Biomedicine
Volume
12
Issue
3
Start Page
132
End Page
140
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Tropical Biomedicine Vol.12 No.3 (2022) , 132-140
Suggested Citation
Sangpairoj K., Settacomkul R., Siangcham T., Meemon K., Niamnont N., Sornkaew N., Tamtin M., Sobhon P., Vivithanaporn P. Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress. Asian Pacific Journal of Tropical Biomedicine Vol.12 No.3 (2022) , 132-140. 140. doi:10.4103/2221-1691.338922 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83798
Title
Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress
Other Contributor(s)
Abstract
Objective: To investigate the effect of the hexane solvent fraction of Halymenia durvillei (HDHE) on triple-negative breast cancer. Methods: The phytochemical profile of HDHE was investigated by GC-MS. The cytotoxicity of HDHE against MDA-MB-231 cells was determined. The apoptotic and autophagic effects of HDHE were analyzed. The expression of molecular markers controlling apoptosis, autophagy, DNA damage, and endoplasmic reticulum (ER) stress was determined. Results: HDHE contains a mixture of fatty acids, mainly hexadecanoic acid. HDHE at a cytotoxic concentration induced apoptotic death of MDA-MB-231 cells through mitochondrial membrane dysfunction, and induction of apoptosis markers, and increased the expression of proteins related to DNA damage response. HDHE also induced the expression of LC-3, a marker of autophagic cell death at a cytotoxic concentration. Moreover, HDHE modulated the expression of ER stress genes. Conclusions: The hexadecanoic acid-enriched extract of Halymenia durvillei promotes apoptosis and autophagy of human triple-negative breast cancer cells. This extract may be further explored as an anticancer agent for triple-negative breast cancer.