In vitro and in vivo evaluation of the layer-by-layer vancomycin with poly(ε-caprolactone) nanosphere-coated Schanz pins for prolonged release
Issued Date
2024-10-01
Resource Type
ISSN
17732247
Scopus ID
2-s2.0-85200805973
Journal Title
Journal of Drug Delivery Science and Technology
Volume
100
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Drug Delivery Science and Technology Vol.100 (2024)
Suggested Citation
Patiyananuwat N., Safarzadeh M., Chartpitak T., Riansuwan K., Ritshima W., Nasongkla N. In vitro and in vivo evaluation of the layer-by-layer vancomycin with poly(ε-caprolactone) nanosphere-coated Schanz pins for prolonged release. Journal of Drug Delivery Science and Technology Vol.100 (2024). doi:10.1016/j.jddst.2024.106029 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/100486
Title
In vitro and in vivo evaluation of the layer-by-layer vancomycin with poly(ε-caprolactone) nanosphere-coated Schanz pins for prolonged release
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Abstract
In this research, the effect of layer-by-layer (spray and dip) coating followed by annealing of vancomycin (VCM) and poly(ε-caprolactone) nanospheres (PCLnp) on Schanz pins for prolonged antibacterial activity was investigated. Nanospheres were prepared using the high-pressure homogenizer method and characterized by both functional groups and particle size. The in vitro release profile revealed that after 28 days, the cumulative release of VCM from the VCM-PCLnp coated pins was 753.68 ± 0.19 μg. The coated pins released VCM at a concentration greater than the minimum inhibitory concentration (MIC) and sustained this release for 28 days. Irritation, hemolysis, sensitization, and cytotoxicity assays confirmed that VCM-PCLnp coated pins were highly biocompatible. Additionally, antibacterial assays against methicillin-resistant Staphylococcus aureus (MRSA) revealed prolonged antibacterial resistance. Specifically, the assays demonstrated effective inhibition of Staphylococcus aureus (S. aureus), including the methicillin-resistant strain. This approach involves coating the implants with antibiotics and polymeric nanospheres using a layer-by-layer (dip and spray) method to prolong their antibacterial activity. The results of this study show great promise for successfully treating implant-associated infections. Consequently, we recommend this method for coating other medical devices.