Identification and expression of gene loci underlying the quantitative trait loci of starch content in cassava
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Issued Date
2024-01-01
Resource Type
ISSN
15427528
eISSN
15427536
Scopus ID
2-s2.0-85193695244
Journal Title
Journal of Crop Improvement
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Crop Improvement (2024)
Suggested Citation
Sawangsalee K., Sraphet S., Srisawad N., Munkongdee T., Svasti S., Bumrung S., Smith D.R., Triwitayakorn K. Identification and expression of gene loci underlying the quantitative trait loci of starch content in cassava. Journal of Crop Improvement (2024). doi:10.1080/15427528.2024.2353094 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/98503
Title
Identification and expression of gene loci underlying the quantitative trait loci of starch content in cassava
Corresponding Author(s)
Other Contributor(s)
Abstract
In this study, a genetic linkage map was developed based on simple sequence repeat (SSR) markers to aid in the identification and expression analysis of gene loci underlying the quantitative trait loci (QTL) of starch content (SC) in cassava (Manihot esculenta). A total of 912 polymorphic loci from 4,515 tested SSRs were mapped into 18 linkage groups. QTL analysis revealed four loci with highly significant associations with starch. Gene annotation was performed, and potential genes related to starch synthesis were identified. Of these, expression of four genes related to starch synthesis (Raffinose synthase [RS], Phosphoenolpyruvate kinase [PK], Glucose-6-phosphate [G6P], and Glycogen synthase kinase 3 [GSK-3]) was investigated by real-time PCR assays. The results found a statistically significant difference (p < 0.05) at 3 months after planting (MAP) in all the tested genes, whereas a higher expression was detected in the low-SC group with specific genotypes of linked markers. A higher level of expression was observed in the RS, PK, and G6P genes at six and nine MAP, indicating that starch accumulation mainly occurs after 3 MAP, during which the root system begins to form. The potential genes identified in this study could provide a better understanding of the starch biosynthesis mechanism, and could be useful for applications in marker-assisted selection to facilitate cassava breeding programs in the future.