Protocol for quantifying N-Myc and global protein translation in neuroblastoma cells using click chemistry on polyvinylidene fluoride membranes
Issued Date
2024-12-20
Resource Type
eISSN
26661667
Scopus ID
2-s2.0-85207343442
Pubmed ID
39396233
Journal Title
STAR Protocols
Volume
5
Issue
4
Rights Holder(s)
SCOPUS
Bibliographic Citation
STAR Protocols Vol.5 No.4 (2024)
Suggested Citation
Chittavanich P., Saengwimol D., Sari A.I.P., Srimongkol A., Kaewkhaw R. Protocol for quantifying N-Myc and global protein translation in neuroblastoma cells using click chemistry on polyvinylidene fluoride membranes. STAR Protocols Vol.5 No.4 (2024). doi:10.1016/j.xpro.2024.103377 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/101850
Title
Protocol for quantifying N-Myc and global protein translation in neuroblastoma cells using click chemistry on polyvinylidene fluoride membranes
Author's Affiliation
Corresponding Author(s)
Other Contributor(s)
Abstract
MYCN amplification is a hallmark of aggressive neuroblastoma, driving N-Myc overexpression and enhancing protein synthesis, making these processes potential therapeutic targets. Here, we present a protocol for quantifying nascent N-Myc and global protein translation in neuroblastoma cells. This protocol describes the steps for labeling nascent proteins and performing an optimized click chemistry reaction directly on the membrane after blotting, enabling high-sensitivity detection and analysis. Adaptable to other proteins of interest, this approach provides valuable insights into neuroblastoma protein synthesis. For complete details on the use and execution of this protocol, please refer to Chittavanich et al.1