tesG expression as a potential clinical biomarker for chronic Pseudomonas aeruginosa pulmonary biofilm infections
Issued Date
2025-12-01
Resource Type
eISSN
17417015
Scopus ID
2-s2.0-105001243879
Journal Title
BMC Medicine
Volume
23
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
BMC Medicine Vol.23 No.1 (2025)
Suggested Citation
Wannigama D.L., Hurst C., Monk P.N., Hartel G., Ditcham W.G.F., Hongsing P., Phattharapornjaroen P., Ounjai P., Torvorapanit P., Jutivorakool K., Luk-in S., Nilgate S., Rirerm U., Tanasatitchai C., Miyanaga K., Cui L., Ragupathi N.K.D., Rad S.M.A.H., Khatib A., Storer R.J., Ishikawa H., Amarasiri M., Charuluxananan S., Leelahavanichkul A., Kanjanabuch T., Higgins P.G., Davies J.C., Stick S.M., Kicic A., Chatsuwan T., Shibuya K., Abe S. tesG expression as a potential clinical biomarker for chronic Pseudomonas aeruginosa pulmonary biofilm infections. BMC Medicine Vol.23 No.1 (2025). doi:10.1186/s12916-025-04009-x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/109379
Title
tesG expression as a potential clinical biomarker for chronic Pseudomonas aeruginosa pulmonary biofilm infections
Author(s)
Wannigama D.L.
Hurst C.
Monk P.N.
Hartel G.
Ditcham W.G.F.
Hongsing P.
Phattharapornjaroen P.
Ounjai P.
Torvorapanit P.
Jutivorakool K.
Luk-in S.
Nilgate S.
Rirerm U.
Tanasatitchai C.
Miyanaga K.
Cui L.
Ragupathi N.K.D.
Rad S.M.A.H.
Khatib A.
Storer R.J.
Ishikawa H.
Amarasiri M.
Charuluxananan S.
Leelahavanichkul A.
Kanjanabuch T.
Higgins P.G.
Davies J.C.
Stick S.M.
Kicic A.
Chatsuwan T.
Shibuya K.
Abe S.
Hurst C.
Monk P.N.
Hartel G.
Ditcham W.G.F.
Hongsing P.
Phattharapornjaroen P.
Ounjai P.
Torvorapanit P.
Jutivorakool K.
Luk-in S.
Nilgate S.
Rirerm U.
Tanasatitchai C.
Miyanaga K.
Cui L.
Ragupathi N.K.D.
Rad S.M.A.H.
Khatib A.
Storer R.J.
Ishikawa H.
Amarasiri M.
Charuluxananan S.
Leelahavanichkul A.
Kanjanabuch T.
Higgins P.G.
Davies J.C.
Stick S.M.
Kicic A.
Chatsuwan T.
Shibuya K.
Abe S.
Author's Affiliation
The Tokyo Foundation for Policy Research
Yamagata Prefectural Central Hospital
Faculty of Science, Mahidol University
UWA Medical School
Yamagata Prefectural University of Health Sciences
Perth Children's Hospital
Jichi Medical University
Curtin University
The University of Queensland
Chulalongkorn University
QIMR Berghofer Medical Research Institute
Chulabhorn Royal Academy
King Chulalongkorn Memorial Hospital
University of Toronto Faculty of Medicine
University of Otago
Faculty of Medicine, Thammasat University
Mahidol University
Thammasat University
National Heart and Lung Institute
Royal Brompton Hospital
The Sheffield Medical School
Uniklinik Köln
Tohoku University
Yamagata University Hospital
Faculty of Medicine, Chulalongkorn University
The University of Sheffield
Christian Medical College, Vellore
The Kids Research Institute Australia
Partner Site Bonn-Cologne
Yamagata Prefectural Central Hospital
Faculty of Science, Mahidol University
UWA Medical School
Yamagata Prefectural University of Health Sciences
Perth Children's Hospital
Jichi Medical University
Curtin University
The University of Queensland
Chulalongkorn University
QIMR Berghofer Medical Research Institute
Chulabhorn Royal Academy
King Chulalongkorn Memorial Hospital
University of Toronto Faculty of Medicine
University of Otago
Faculty of Medicine, Thammasat University
Mahidol University
Thammasat University
National Heart and Lung Institute
Royal Brompton Hospital
The Sheffield Medical School
Uniklinik Köln
Tohoku University
Yamagata University Hospital
Faculty of Medicine, Chulalongkorn University
The University of Sheffield
Christian Medical College, Vellore
The Kids Research Institute Australia
Partner Site Bonn-Cologne
Corresponding Author(s)
Other Contributor(s)
Abstract
Background: Pseudomonas aeruginosa infections in the lungs affect millions of children and adults worldwide. To our knowledge, no clinically validated prognostic biomarkers for chronic pulmonary P. aeruginosa infections exist. Therefore, this study aims to identify potential prognostic markers for chronic P. aeruginosa biofilm lung infections. Methods: Here, we screened the expression of 11 P. aeruginosa regulatory genes (tesG, algD, lasR, lasA, lasB, pelB, phzF, rhlA, rsmY, rsmZ, and sagS) to identify associations between clinical status and chronic biofilm infection. Results: RNA was extracted from 210 sputum samples from patients (n = 70) with chronic P. aeruginosa lung infections (mean age; 29.3–56.2 years; 33 female). Strong biofilm formation was correlated with prolonged hospital stays (212.2 days vs. 44.4 days) and increased mortality (46.2% (18)). Strong biofilm formation is associated with increased tesG expression (P = 0.001), influencing extended intensive care unit (P = 0.002) or hospitalisation stays (P = 0.001), pneumonia risk (P = 0.006), and mortality (P = 0.001). Notably, tesG expression is linked to the modulation of systemic and sputum inflammatory responses and predicts biofilm biomass. Conclusions: This study provides the first clinical dataset of tesG expression levels as a predictive biomarker for chronic P. aeruginosa pulmonary infections.