Nanocomposite-based all-in-one electrode coating towards affinity-based electrochemical biosensing platform
10
Issued Date
2026-06-01
Resource Type
ISSN
13858947
Scopus ID
2-s2.0-105036050831
Journal Title
Chemical Engineering Journal
Volume
537
Rights Holder(s)
SCOPUS
Bibliographic Citation
Chemical Engineering Journal Vol.537 (2026)
Suggested Citation
Tungtrakarnkul P., Kumar R.K.R., Bharti A.M., Lawaniya S.D., Hu W.C., Chuang C.H., Huang S.F., Wu K.C.W., Lertanantawong B. Nanocomposite-based all-in-one electrode coating towards affinity-based electrochemical biosensing platform. Chemical Engineering Journal Vol.537 (2026). doi:10.1016/j.cej.2026.175986 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116392
Title
Nanocomposite-based all-in-one electrode coating towards affinity-based electrochemical biosensing platform
Corresponding Author(s)
Other Contributor(s)
Abstract
The commercialization of affinity-based electrochemical point-of-care devices remain constrained by their reliance on external redox mediators and the longstanding challenge of simultaneously achieving high signal sensitivity and antifouling capability. Herein, we report a hierarchical nanocomposite-based all-in-one electrode coating that strategically integrates intrinsic redox activity, antifouling capability, and high conductivity into a unified, drop-castable interface. The nanocomposite comprises of redox-active ferrocene-functionalized Ce-UiO-66-NH₂ metal-organic framework (Ce-MOF-Fc) and highly conductive polyaniline nanowires (PANI NWs) embedded within glutaraldehyde-crosslinked bovine serum albumin (GB) matrix. The GB matrix suppresses nonspecific adsorption and retains 84% signal after extended exposure to fouling biofluids. Its recessed, open-pore morphology and percolated PANI NWs network enable efficient charge transport and amplifies the intrinsic redox signal of Ce-MOF-Fc. Furthermore, the nanocomposite supports facile covalent immobilization of amine-terminated biorecognition elements (e.g., aptamers, antibodies) via 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride/N-hydroxy succinimide (EDC/NHS) activation of bovine serum albumin (BSA) carboxyl groups. As a proof-of-concept, an epithelial cell adhesion molecule (EpCAM)-specific aptamer (Apt-NH₂) was designed and immobilized for label-free detection of M D Anderson-Metastatic Breast −231 (MDA-MB-231) breast cancer cells. The biosensor exhibited linearity from 5 to 800 cells/mL, a detection limit of 5 cells/mL, high reproducibility, and excellent specificity in simulated serum and urine. This modular and versatile platform offers a valuable blueprint for next-generation electrochemical affinity biosensors with broad applicability in real-world biomedical diagnostics.