Pragmatic Applications and Universality of DNA Barcoding for Substantial Organisms at Species Level: A Review to Explore a Way Forward
Issued Date
2022-01-01
Resource Type
ISSN
23146133
eISSN
23146141
Scopus ID
2-s2.0-85123746219
Pubmed ID
35059459
Journal Title
BioMed Research International
Volume
2022
Rights Holder(s)
SCOPUS
Bibliographic Citation
BioMed Research International Vol.2022 (2022)
Suggested Citation
Ahmed S., Ibrahim M., Nantasenamat C., Nisar M.F., Malik A.A., Waheed R., Ahmed M.Z., Ojha S.C., Alam M.K. Pragmatic Applications and Universality of DNA Barcoding for Substantial Organisms at Species Level: A Review to Explore a Way Forward. BioMed Research International Vol.2022 (2022). doi:10.1155/2022/1846485 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83928
Title
Pragmatic Applications and Universality of DNA Barcoding for Substantial Organisms at Species Level: A Review to Explore a Way Forward
Other Contributor(s)
Abstract
DNA barcodes are regarded as hereditary succession codes that serve as a recognition marker to address several queries relating to the identification, classification, community ecology, and evolution of certain functional traits in organisms. The mitochondrial cytochrome c oxidase 1 (CO1) gene as a DNA barcode is highly efficient for discriminating vertebrate and invertebrate animal species. Similarly, different specific markers are used for other organisms, including ribulose bisphosphate carboxylase (rbcL), maturase kinase (matK), transfer RNA-H and photosystem II D1-ApbsArabidopsis thaliana (trnH-psbA), and internal transcribed spacer (ITS) for plant species; 16S ribosomal RNA (16S rRNA), elongation factor Tu gene (Tuf gene), and chaperonin for bacterial strains; and nuclear ITS for fungal strains. Nevertheless, the taxon coverage of reference sequences is far from complete for genus or species-level identification. Applying the next-generation sequencing approach to the parallel acquisition of DNA barcode sequences could greatly expand the potential for library preparation or accurate identification in biodiversity research. Overall, this review articulates on the DNA barcoding technology as applied to different organisms, its universality, applicability, and innovative approach to handling DNA-based species identification.