Expression and characterization of the antimicrobial peptide gambicin from culex quinquefasciatus
Issued Date
2013
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Apanchanid Thepouyporn, อาพันธ์ชนิด เทพอวยพร, Phanthila Sirichaiyakul, ภัณฑิลา ศิริไชยยากุล, Rungarun Suthangkornkul, รุ่งอรุณ สุธางค์กรกุล, Okabayashi Tamaki, Yoshiharu Matsuura, Naokazu Takeda, Dumrongkiet Arthan, ดำรงเกียรติ อาจหาญ (2013). Expression and characterization of the antimicrobial peptide gambicin from culex quinquefasciatus. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63337
Title
Expression and characterization of the antimicrobial peptide gambicin from culex quinquefasciatus
Abstract
Antimicrobial peptides (AMPs), which can be found in many organisms, are generally low molecular
weight peptides containing less than 100 amino acid residues. Due to ineffective conventional
antibiotic treatments causing numerous mortalities of infected patients in the world, AMPs are an
interesting approach to the development of novel therapeutics against pathogens, particularly antibioticresistant
bacteria, because they are safe, non-toxic to mammals, no bacterial resistance and showing broadspectrum
antimicrobial activity. Interestingly, an antimicrobial peptide gambicin isolated from An. gambiae
cell lines possess antimicrobial activity against gram-positive and -negative bacteria, filamentous fungi, and
Plasmodium species. However, the isolation of Culex gambicin and expression of recombinant mosquito
gambicins remain unreported. This study aimed to express recombinant Culex gambicin in Pichia pastoris and/
or baculovirus expression system, and successfully cloned and expressed. SDS-PAGE of Culex gambicin in Pichia
pastoris revealed an obvious protein band with a molecular weight of 7.0 kDa, indicating high production of
recombinant Culex gambicin. Testing of antimicrobial activity against E. coli DH5a strain by agar-well diffusion
assay exhibited no activity. A refolding approach to the recovery of antimicrobial peptides is required for
further studies, while the expression of recombinant gambicin in the baculovirus expression system is on the process which will be also discussed.
Description
Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine 11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.146.