Thrombopoietin-independent generation of platelet-like particles from megakaryoblastic cells
Issued Date
2023-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-85180251075
Pubmed ID
38110522
Journal Title
Scientific Reports
Volume
13
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.13 No.1 (2023)
Suggested Citation
Nunthanasup N., Ketprasit N., Noulsri E., Palasuwan A., Combes V., Kulkeaw K., Palasuwan D. Thrombopoietin-independent generation of platelet-like particles from megakaryoblastic cells. Scientific Reports Vol.13 No.1 (2023). doi:10.1038/s41598-023-50111-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/95824
Title
Thrombopoietin-independent generation of platelet-like particles from megakaryoblastic cells
Corresponding Author(s)
Other Contributor(s)
Abstract
The use of megakaryoblastic leukemia MEG-01 cells can help reveal the mechanisms of thrombopoiesis. However, conventional in vitro activation of platelet release from MEG-01 cells requires thrombopoietin, which is costly. Here, we aim to develop a more straightforward and affordable method. Synchronization of the MEG-01 cells was initially performed using serum-free culture, followed by spontaneous cell differentiation in the presence of serum. Different stages of megakaryoblast differentiation were classified based on cell morphology, DNA content, and cell cycle. The MEG-01 cells released platelet-like particles at a level comparable to that of the thrombopoietin-activated MEG-01 cells. The platelet-like particles were distinguishable from PLP-derived extracellular vesicles and could express P-selectin following ADP activation. Importantly, the platelet-like particles induced fibrin clotting in vitro using platelet-poor plasma. Therefore, this thrombopoietin-independent cell synchronization method is an effective and straightforward method for studying megakaryopoiesis and thrombopoiesis.