Studies on fungal lipases for interesterification of palm oil
3
Issued Date
2023
Copyright Date
1990
Language
eng
File Type
application/pdf
No. of Pages/File Size
x, 110 leaves : ill.
Access Rights
restricted access
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Biochemistry))--Mahidol University, 1990
Suggested Citation
Napawan Promkhatkaew Studies on fungal lipases for interesterification of palm oil. Thesis (M.Sc. (Biochemistry))--Mahidol University, 1990. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/90450
Title
Studies on fungal lipases for interesterification of palm oil
Alternative Title(s)
การศึกษาเอนไซม์ไลเปสจากเชื้อราเพื่อใช้เร่งอินเตอร์เอสเตอริฟิเคชั่นในน้ำมันปาล์ม
Author(s)
Abstract
Attempts were made to find out efficient lipase producers from five strains of fungi, namely Aspergillus niger 3240, A. niger MUCA, A. niger 3092, Mucor TISTR and Rhizopus delemar. Of these, R. delemar grown in submerged culture produced lipase of high activity (~800 unit/ml) and A. niger 3240 grown on wheat bran-based semi-solid culture also produced subtantial amount of lipase (1400 unit/g medium). Crude lipase in the culture extract of A. niger 3240 had optimal pH at 5, optimal temperature at 30 C and heat stability up to 60 C. The enzyme was purified by ammonium sufate preciptation followed by DEAE-cellulose and Sephadex G-100 column chromatography. The yield obtained was 43% with 16 fold purification. The partially purified lipase was immobilized on celite for being used in intereste-rification reaction of palm oil with [1-14C] -stearic acid. The celite-adsorbed lipase had pH stability in the range of 3-9 and catalyzed incorporation of [1-14C] -stearic acid into triglyceride of palm oil at optimum temperature 45 C, optimum pH 3-5 and was favorable to catalyze interesterification of triglycerides having long-chain fatty acid in water-saturated n-hexane. The immobilized lipase was shown to be able to catalyze interesterification of palm oil with stearic, linoleic and arachidonic acids. The interesterified product analyzed by gas liquid chromatography showed the replacement of palmityl and oleoyl moieties in trigly-cerides of palm poil with the added fatty acids. Further work is required to improve efficiency of lipase-catalyzed interesterification of paim oil.
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Biochemistry
Degree Grantor(s)
Mahidol University