New xanthone and chemical constituents from the aerial parts of Mallotus glomerulatus and their cytotoxicity in MCF-7 and MDA-MB-231 breast cancer cells
3
Issued Date
2025-01-01
Resource Type
eISSN
18487718
Scopus ID
2-s2.0-105026188227
Journal Title
Admet and Dmpk
Volume
13
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
Admet and Dmpk Vol.13 No.5 (2025)
Suggested Citation
Chabang N., Wongwitayasombat C., Tuchinda P., Munyoo B., Kangwanrangsan N., Hongeng S., Nutho B., Charoensutthivarakul S., Kanjanasirirat P. New xanthone and chemical constituents from the aerial parts of Mallotus glomerulatus and their cytotoxicity in MCF-7 and MDA-MB-231 breast cancer cells. Admet and Dmpk Vol.13 No.5 (2025). doi:10.5599/admet.2901 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/113789
Title
New xanthone and chemical constituents from the aerial parts of Mallotus glomerulatus and their cytotoxicity in MCF-7 and MDA-MB-231 breast cancer cells
Corresponding Author(s)
Other Contributor(s)
Abstract
Background and purpose: Breast cancer remains a significant global health burden, especially in low-resource settings where standard therapies are limited. This study aimed to explore Mallotus glomerulatus, a lesser-known Thai medicinal plant, as a potential source of novel anti-breast cancer agents. Experimental approach: A phytochemical investigation of M. glomerulatus resulted in the isolation and structural characterization of a novel xanthone (Compound 1) and cleistanthin A (Compound 10) using UV, IR, NMR, and HRMS techniques. Cytotoxicity of the compounds was evaluated in vitro against MCF-7 (ER-positive) and MDA-MB-231 (triple-negative) breast cancer cell lines, along with HepG2 liver cells. Molecular docking studies were conducted to assess their interaction with vacuolar H<sup>+</sup>-ATPase (V-ATPase). Key results: Compound 1 demonstrated selective cytotoxicity toward MCF-7 cells, whereas cleistanthin A exhibited potent cytotoxicity against both breast cancer lines, with nanomolar IC50 values and a high selectivity index (>100) for MDA-MB-231 compared to HepG2 cells. Docking analysis revealed favourable binding of both compounds at the a–c subunit interface of V-ATPase, suggesting a mechanism involving proton pump inhibition and lysosomal dysfunction. Conclusion: The findings highlight M. glomerulatus, particularly cleistanthin A, as a promising source of safe and affordable anti-breast cancer compounds with potential therapeutic value. Further studies on the mechanism and in vivo efficacy are warranted.