Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA
Issued Date
2023-01-01
Resource Type
ISSN
10643745
eISSN
19406029
Scopus ID
2-s2.0-85141003992
Pubmed ID
36306078
Journal Title
Methods in Molecular Biology
Volume
2555
Start Page
51
End Page
72
Rights Holder(s)
SCOPUS
Bibliographic Citation
Methods in Molecular Biology Vol.2555 (2023) , 51-72
Suggested Citation
Tansirichaiya S., Hutton W., Roberts A.P. Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA. Methods in Molecular Biology Vol.2555 (2023) , 51-72. 72. doi:10.1007/978-1-0716-2795-2_4 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/82340
Title
Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Antimicrobial resistance (AMR) is an increasingly important global challenge for healthcare systems as well as agricultural food production systems. Our ability to prepare for, and respond to, emerging AMR threats is dependent on our knowledge of genes able to confer AMR that are circulating within various environmental, animal, and human microbiomes. Targeted, sequence-specific, detection of AMR genes and functional resistance assays, described here, carried out on metagenomic DNA gives us unique insights into the presence of AMR genes and how these are associated with mobile genetic elements that may be responsible for their dissemination and can also provide important information about the mechanisms of resistance underpinning the phenotype.