Functional and Immunologic Mapping of Domains of the Reticulocyte-Binding Protein Plasmodium vivax PvRBP2a
Issued Date
2024-09-23
Resource Type
eISSN
15376613
Scopus ID
2-s2.0-85204819207
Pubmed ID
38441336
Journal Title
The Journal of infectious diseases
Volume
230
Issue
3
Start Page
e737
End Page
e742
Rights Holder(s)
SCOPUS
Bibliographic Citation
The Journal of infectious diseases Vol.230 No.3 (2024) , e737-e742
Suggested Citation
Tay M.Z., Tang W., Lee W.C., Ong A.S.M., Novera W., Malleret B., Carissimo G., Chacko A.M., El-Sahili A., Lescar J., Fan Y., McGready R.M., Chu C.S., Chan J.K.Y., Ng L.F.P., Russell B., Nosten F., Rénia L. Functional and Immunologic Mapping of Domains of the Reticulocyte-Binding Protein Plasmodium vivax PvRBP2a. The Journal of infectious diseases Vol.230 No.3 (2024) , e737-e742. e742. doi:10.1093/infdis/jiae111 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/101433
Title
Functional and Immunologic Mapping of Domains of the Reticulocyte-Binding Protein Plasmodium vivax PvRBP2a
Author's Affiliation
A-Star, Infectious Disease Lab
Mahidol Oxford Tropical Medicine Research Unit
Lee Kong Chian School of Medicine
Duke-NUS Medical School
Universiti Malaya
NUS Yong Loo Lin School of Medicine
University of Otago
KK Women's And Children's Hospital
Nuffield Department of Medicine
Nanyang Technological University
Immunology Translational Research Programme
Mahidol Oxford Tropical Medicine Research Unit
Lee Kong Chian School of Medicine
Duke-NUS Medical School
Universiti Malaya
NUS Yong Loo Lin School of Medicine
University of Otago
KK Women's And Children's Hospital
Nuffield Department of Medicine
Nanyang Technological University
Immunology Translational Research Programme
Corresponding Author(s)
Other Contributor(s)
Abstract
We previously described a novel Plasmodium vivax invasion mechanism into human reticulocytes via the PvRBP2a-CD98 receptor-ligand pair. Using linear epitope mapping, we assessed the PvRBP2a epitopes involved in CD98 binding and recognized by antibodies from patients who were infected. We identified 2 epitope clusters mediating PvRBP2a-CD98 interaction. Cluster B (PvRBP2a431-448, TAALKEKGKLLANLYNKL) was the target of antibody responses in humans infected by P vivax. Peptides from each cluster were able to prevent live parasite invasion of human reticulocytes. These results provide new insights for development of a malaria blood-stage vaccine against P vivax.