Alteration of zonula occludens-1 (zo-1) in acute kidney injury in severe malaria
1
Issued Date
2013
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Benjamas Wichapoon, เบญจมาศ วิชาพูล, Chuchard Punsawad, Riganti, Mario, Parnpen Viriyavejakul, พรรณเพ็ญ วิริยเวชกุล (2013). Alteration of zonula occludens-1 (zo-1) in acute kidney injury in severe malaria. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63191
Title
Alteration of zonula occludens-1 (zo-1) in acute kidney injury in severe malaria
Other Contributor(s)
Abstract
Background: Zonula occludens-1 (ZO-1) proteins are scaffolding proteins providing the permeability
barrier at the cytoplasmic surface of intercellular junctions. Maintenance of cell junction is important
to balance the fluids and substances between compartments. In Plasmodium falciparum malaria, AKI is a
major complication causing fatality and it will be valuable to investigate Z0-1 in kidney tissues and endothelial
cells (ECs) in severe P. falciparum malaria. Methods: Using immunohistochemistry, paraffin-embedded kidney tissues of fetal malaria were stained
with rabbit polyclonal anti-ZO-1. For immunofluorescence, ZO-1 was demonstrated in ECs-malaria parasite coculture.
The expression of ZO-1 and degree of intensity were compared. Results: Significant decreased in ZO-1 expression of AKI and non-AKI cases was observed in
comparison to control kidney samples (21.8 ± 4.3, 85.9 ± 10.8 vs 171.4 ± 17.5; p 0.001, p = 0.006, respectively).
AKI cases also showed significant decreased in ZO-1 expression as compared to non-AKI cases (p 0.001).
Immunofluorescence study showed significant alteration of ECs tight junction in malaria cases when
compared with control. Conclusions: The loss of protein tight junction (ZO-1) is associated with AKI. This is confirmed by
alteration of ECs tight junction demonstrated in ECs-malaria parasite co-culture system.
Description
Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.141.