Yield and Properties of Collagen from Nile Tilapia (Oreochromis niloticus) Scales: Effects of Ultrasonic Pretreatment on Pepsin-Aided Extraction
Issued Date
2026-02-02
Resource Type
eISSN
25869396
Scopus ID
2-s2.0-105029749309
Journal Title
Current Applied Science and Technology
Volume
26
Issue
2
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SCOPUS
Bibliographic Citation
Current Applied Science and Technology Vol.26 No.2 (2026)
Suggested Citation
Kittiphattanabawon P., Kishimura H., Benjakul S., Visessanguan W. Yield and Properties of Collagen from Nile Tilapia (Oreochromis niloticus) Scales: Effects of Ultrasonic Pretreatment on Pepsin-Aided Extraction. Current Applied Science and Technology Vol.26 No.2 (2026). doi:10.55003/cast.2025.266314 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/115164
Title
Yield and Properties of Collagen from Nile Tilapia (Oreochromis niloticus) Scales: Effects of Ultrasonic Pretreatment on Pepsin-Aided Extraction
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Abstract
The yield and characteristics of collagen from Nile tilapia scale pretreated with ultrasound at 40 kHz for 2 h before pepsin-aided extraction for 12-72 h (US collagen) were investigated compared to collagen from the scales without ultrasonic pretreatment (non-US collagen). Both collagens' yields increased with prolonged extraction time (P<0.05). Nevertheless, the yield of ultrasound-pretreated collagen (2.20-4.31%) was approximately 2 times greater than that of collagen without ultrasonic pretreatment (1.06-2.03%) (P<0.05). The amino acid compositions of both collagens were comparable, consisting mainly of glycine, alanine, proline, and hydroxyproline (329-330, 115,119, 114-116, 85 residues per 1000 residues, respectively), and both were classified as type I collagen. Moreover, the thermal transition temperatures (39.38-39.43°C) and enthalpy (0.55 J/g) were comparable between both collagens (P>0.05). Analysis of the FTIR spectra indicated that the ultrasonication pretreatment of the scale before the collagen did not alter the triple-helical structure of the collagen. Therefore, pretreatment of the Nile tilapia scales with ultrasonication before the pepsin-aided process could increase yield without significantly affecting the characteristics and triple-helical structure of the collagen.