Process development of sugar beet enzymatic hydrolysis with enzyme recycling for soluble sugar production
Issued Date
2022-08-01
Resource Type
ISSN
16157591
eISSN
16157605
Scopus ID
2-s2.0-85133282700
Pubmed ID
35779112
Journal Title
Bioprocess and Biosystems Engineering
Volume
45
Issue
8
Start Page
1297
End Page
1309
Rights Holder(s)
SCOPUS
Bibliographic Citation
Bioprocess and Biosystems Engineering Vol.45 No.8 (2022) , 1297-1309
Suggested Citation
Chen Y., Aramrueang N., Zicari S., Zhang R. Process development of sugar beet enzymatic hydrolysis with enzyme recycling for soluble sugar production. Bioprocess and Biosystems Engineering Vol.45 No.8 (2022) , 1297-1309. 1309. doi:10.1007/s00449-022-02742-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83661
Title
Process development of sugar beet enzymatic hydrolysis with enzyme recycling for soluble sugar production
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Abstract: Enzymatic hydrolysis of sugar beets for achieving liquefaction and sugar release is a critical step for beet-ethanol production. An enzyme recycling process was developed in this study to reduce the economic uncertainty raised by the high costs of enzymes by reducing the fresh enzyme usage. A mixture of cellulases and pectinases was used in the beet hydrolysis. The hydrolysate was centrifuged and then processed through a 50 kDa molecular weight cut-off polyethersulfone membrane to recover enzymes from the liquid. Liquid enzyme recycling with 50% fresh enzyme addition achieved a similar liquefaction extent and sugar yield compared to the positive control with 100% fresh enzyme. Solid enzyme recycling showed a lower liquefaction efficiency, requiring at least 75% of fresh enzyme addition for a comparable liquefaction extent. Five sequential batches of hydrolysis with liquid enzyme recycling were successfully conducted to hydrolyze sugar beets with similar liquefaction extents and sugar yields.