Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria
Issued Date
2022-09-01
Resource Type
eISSN
19326203
Scopus ID
2-s2.0-85137136049
Pubmed ID
36048830
Journal Title
PLoS ONE
Volume
17
Issue
9 September
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE Vol.17 No.9 September (2022)
Suggested Citation
Htoo H.H., Tuyet N.N.T., Nakprasit K., Aonbangkhen C., Chaikeeratisak V., Chavasiri W., Nonejuie P. Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria. PLoS ONE Vol.17 No.9 September (2022). doi:10.1371/journal.pone.0273614 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/86482
Title
Mansonone G and its derivatives exhibit membrane permeabilizing activities against bacteria
Author's Affiliation
Other Contributor(s)
Abstract
In an era where the rate of bacteria evolving to be resistant to clinically-used antibiotics far exceeds that of antibiotic discovery, the search for new sources of antibacterial agents has expanded tremendously. In recent years, interest in plant-based natural products as promising sources of antibacterial agents has taken an upward trend. Mansonones, botanically-derived naphthoqionones, having many uses in Asian traditional medicine-including anti-infective roles-have sparked interest as a possible source of antibacterial agents. Here, we show that mansonone G, extracted from Mansonia gagei Drumm. heartwoods, possessed antibacterial activities towards Bacillus subtilis, Staphylococcus aureus and Escherichia coli lptD4213, inhibiting the growth of the bacteria at 15.6 μM, 62.5 μM and 125 μM, respectively. Fourteen derivatives of mansonone G were synthesized successfully and were found to have a similar antibacterial spectrum to that of the parent compound, with some derivatives possessing improved antibacterial activities. Bacterial cytological profiling analysis showed that mansonone G harbors membrane permeabilizing activities against B. subtilis and E. coli lptD4213. Temporal analysis of SYTOX Green staining among individual cells showed that mansonone G rapidly permeabilized bacterial membrane within 10 min, with SYTOX Green intensity reaching 13-fold above that of the control. Collectively, these findings highlight the importance of mansonone G and its derivatives as potential antibacterial agents, paving the way for further modifications in order to improve their antibacterial spectrum.