Efficient Generation of iPSC-Derived Hematoendothelial Progenitors and Specification Toward T cell Lineage
1
Issued Date
2022-01-01
Resource Type
ISSN
10643745
eISSN
19406029
Scopus ID
2-s2.0-85132455752
Pubmed ID
33755900
Journal Title
Methods in Molecular Biology
Volume
2454
Start Page
423
End Page
442
Rights Holder(s)
SCOPUS
Bibliographic Citation
Methods in Molecular Biology Vol.2454 (2022) , 423-442
Suggested Citation
Suwanpitak S., Promnakhon N., Netsrithong R., Wattanapanitch M. Efficient Generation of iPSC-Derived Hematoendothelial Progenitors and Specification Toward T cell Lineage. Methods in Molecular Biology Vol.2454 (2022) , 423-442. 442. doi:10.1007/7651_2021_355 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83901
Title
Efficient Generation of iPSC-Derived Hematoendothelial Progenitors and Specification Toward T cell Lineage
Author's Affiliation
Other Contributor(s)
Abstract
One of the major obstacles for adoptive cell transfer (ACT) of T cells is the loss of effector function and proliferative ability of isolated antigen-specific T cells after prolonged ex vivo expansion. To overcome this issue, induced pluripotent stem cells (iPSCs), which have unlimited proliferation and differentiation potential, can be used to generate a large number of antigen-specific T cells. Here, we describe an efficient differentiation protocol for the generation of cytotoxic CD8+ T cells from human T cell-derived iPSCs (T-iPSCs). The protocol consists of three main steps including differentiation of T-iPSCs toward hematoendothelial progenitors (HEPs), co-culture of HEPs with OP9-DL1 cells, and stimulation of T cell receptor (TCR) signaling to obtain CD8 single-positive (SP) T cells. This culture system is simple and efficient; therefore, will offer a powerful tool for studying T cell development and applications in adoptive immunotherapy.