Nucleus-forming jumbophage PhiKZ therapeutically outcompetes non-nucleus-forming jumbophage Callisto
Issued Date
2024-05-17
Resource Type
eISSN
25890042
Scopus ID
2-s2.0-85191959912
Journal Title
iScience
Volume
27
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
iScience Vol.27 No.5 (2024)
Suggested Citation
Naknaen A., Samernate T., Saeju P., Nonejuie P., Chaikeeratisak V. Nucleus-forming jumbophage PhiKZ therapeutically outcompetes non-nucleus-forming jumbophage Callisto. iScience Vol.27 No.5 (2024). doi:10.1016/j.isci.2024.109790 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98275
Title
Nucleus-forming jumbophage PhiKZ therapeutically outcompetes non-nucleus-forming jumbophage Callisto
Author's Affiliation
Corresponding Author(s)
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Abstract
With the recent resurgence of phage therapy in modern medicine, jumbophages are currently under the spotlight due to their numerous advantages as anti-infective agents. However, most significant discoveries to date have primarily focused on nucleus-forming jumbophages, not their non-nucleus-forming counterparts. In this study, we compare the biological characteristics exhibited by two genetically diverse jumbophages: 1) the well-studied nucleus-forming jumbophage, PhiKZ; and 2) the newly discovered non-nucleus-forming jumbophage, Callisto. Single-cell infection studies further show that Callisto possesses different replication machinery, resulting in a delay in phage maturation compared to that of PhiKZ. The therapeutic potency of both phages was examined in vitro and in vivo, demonstrating that PhiKZ holds certain superior characteristics over Callisto. This research sheds light on the importance of the subcellular infection machinery and the organized progeny maturation process, which could potentially provide valuable insight in the future development of jumbophage-based therapeutics.