Antimicrobial Properties of Ocimum Species: An In Vitro Study
2
Issued Date
2022-11-01
Resource Type
ISSN
22310762
eISSN
22501002
Scopus ID
2-s2.0-85146566565
Journal Title
Journal of International Society of Preventive and Community Dentistry
Volume
12
Issue
6
Start Page
596
End Page
602
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of International Society of Preventive and Community Dentistry Vol.12 No.6 (2022) , 596-602
Suggested Citation
Chanthaboury M., Choonharuangdej S., Shrestha B., Srithavaj T. Antimicrobial Properties of Ocimum Species: An In Vitro Study. Journal of International Society of Preventive and Community Dentistry Vol.12 No.6 (2022) , 596-602. 602. doi:10.4103/jispcd.JISPCD_155_22 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84422
Title
Antimicrobial Properties of Ocimum Species: An In Vitro Study
Author's Affiliation
Other Contributor(s)
Abstract
Objective: This study aimed to determine the antimicrobial activity of ethanol-extracts obtained from Ocimum gratissimum L. (clove or African basil, Lamiaceae) and O. santum L. (holy basil) against some microorganisms present in oral cavity related to either medical or dental disease. Materials and Methods: Antimicrobial properties of both ethanol-extracts of Ocimum species against Streptococcus mutans KPSK2, S. pyogenes ATCC 19615, Staphylococcus aureus ATCC 16794, and Candida albicans ATCC 10231 were primarily determined by agar disk diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal or fungicidal concentration (MBC or MFC) of these herbal extracts were further determined by broth micro-dilution method. Results: Ethanol-extracts of O. sanctum L. and O. gratissimum L. inhibited the growth of all tested microorganisms in various degrees ranging from the strongest antimicrobial activity of O. sanctum against S. pyogenes [MIC at 0.19% (w/v); MBC at 0.78% (w/v)] to the least inhibitory activity of O. gratissimum against C. albicans [MIC at 12.5% (w/v); undetectable MFC]. The ethanol-extract of O. sanctum showed stronger antimicrobial property against the tested bacteria and fungus than O. gratissimum. The ethanol-extracts of both Ocimum species showed stronger antibacterial than antifungal activity. However, the ethanol-extract of O. gratissimum even at a high concentration of 50% (w/v) was unable to eliminate the tested fungus. Conclusion: Ethanol-extracts of Ocimum species contain effective antibacterial and antifungal properties that may be beneficial for further development of antimicrobial agents in medical and dental fields.