Effect of vitamin E supplementation on cholesterol metabolism in rabbit
2
Issued Date
2024
Copyright Date
1989
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
v, 131 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Biochemistry))--Mahidol University, 1989
Suggested Citation
Chopaga Phonpanichrasamee Effect of vitamin E supplementation on cholesterol metabolism in rabbit. Thesis (M.Sc. (Biochemistry))--Mahidol University, 1989. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/99813
Title
Effect of vitamin E supplementation on cholesterol metabolism in rabbit
Alternative Title(s)
ผลของวิตามินดีต่อเมตาบอลิสมของคลอเลสเตอรอลในกระต่าย
Author(s)
Advisor(s)
Abstract
Attempts have been made in this study to confirm the hypocholesterolemic effect of vitamin E in rabbits induced to have hypercholesterolemia by feeding with a high-cholesterol diet and to find out the mechanism by which vitamin E exerts this effect in cholesterol-feeding rabbits. The experiments were carried out by feeding rabbits with a basal diet added with 0.25 and 0.5 % cholesterol, and supplemented with corn oil or with vitamin E of 2100 mg/week for 8 weeks. Rabbits fed the high cholesterol diet with only corn oil supplement showed increased plasma cholesterol of up to 1,000 mg % at the end of feeding period, whereas vitamin E supplementation reduced plasma cholesterol after 4 weeks of feeding by approximately 50 % at the end of feeding period. The hypocholesterolemic effect of vitamin E was observed in cholesterol associated with VLDL+LDL but not HDL fractions. In liver microsomes, the activity of cholesterol 7α-hydroxylase increased 4-5 folds in rabbits fed the high-cholesterol diet with vitamin E supplement compared to those with corn oil supplement. The increased activity of 7α-hydroxylase in vitamin E-supplemented rabbits was consistent to the increase in bile acid concentration in bile of these rabbits as compared to that of the corresponding corn oil supplemented rabbits. Vitamin E supplementation showed no effect on the free cholesterol nor the cholesteryl ester content in liver, but significantly decreased cholesterol content in liver microsomes of vitamin E supplemented as compared with that of vitamin E supplemented as compared with that of corn oil-supplemented rabbits. The cholesterol and phospholipid ratios in liver and bile of vitamin E-supplemented and corn oil supplemented rabbits were not significantly different whereas this ratio in liver microsomes of vitamin E supplemented was slightly lower than that of corn oil supplemented rabbits. Moreover, vitamin E reduced lithogenicity of bile from rabbits fed the high-cholesterol diets, although gallstone was not found in any rabbits. The results observed in this study confirmed suggest that the hypocholesterolemic effect of vitamin E in cholesterol-feeding rabbits may partially involve the increase in conversion of cholesterol to bile acids as the consequence of increased cholesterol 7α-hydroxylase activity. The latter effect of vitamin E may be clinically useful for gallstone prevention.
Description
Biochemistry (Mahidol University 1989)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Biochemistry
Degree Grantor(s)
Mahidol University