Red sticky rice (Oryza sativa L.) bran extract attenuates cellular oxidative stress in human hepatocellular carcinoma cell line via Nrf-2 and HO-1 pathway
Issued Date
2024-10-01
Resource Type
ISSN
15965996
eISSN
15969827
Scopus ID
2-s2.0-85209574454
Journal Title
Tropical Journal of Pharmaceutical Research
Volume
23
Issue
10
Start Page
1617
End Page
1622
Rights Holder(s)
SCOPUS
Bibliographic Citation
Tropical Journal of Pharmaceutical Research Vol.23 No.10 (2024) , 1617-1622
Suggested Citation
Morchang A., Malakar S., Aluksanasuwan S., Somsuan K., Munkong N., Vongthoung K. Red sticky rice (Oryza sativa L.) bran extract attenuates cellular oxidative stress in human hepatocellular carcinoma cell line via Nrf-2 and HO-1 pathway. Tropical Journal of Pharmaceutical Research Vol.23 No.10 (2024) , 1617-1622. 1622. doi:10.4314/tjpr.v23i10.4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/102191
Title
Red sticky rice (Oryza sativa L.) bran extract attenuates cellular oxidative stress in human hepatocellular carcinoma cell line via Nrf-2 and HO-1 pathway
Corresponding Author(s)
Other Contributor(s)
Abstract
Purpose: To investigate the molecular mechanism underlying the antioxidant effect of red sticky rice bran extract (RRBE; a pigmented strain of Oryza sativa L.) in human hepatocellular carcinoma cell line. Methods: Human hepatocellular carcinoma HuH-7 cells were treated with the ethanol extract of RRBE in the presence or absence of EX-537 (Sirtuin 1 inhibitor), dexamethasone (NF-κB inhibitor), brusatol (Nrf-2 inhibitor), or HO-1 inh (HO-1 inhibitor) before exposure to hydrogen peroxide-induced oxidative stress. Intracellular ROS and glutathione levels were assessed using CellROX™ green reagent and GSH-Glo™ glutathione assay, respectively. Levels of nuclear factor erythroid 2-related factor 2 (Nrf-2) and expression of heme oxygenase-1 (HO-1) were determined using immunofluorescent assay and real-time polymerase chain reaction (RT-PCR), respectively. Results: None of the tested inhibitors affected the ability of RRBE to reduce the level of ROS. Treatment with RRBE significantly decreased intracellular ROS and glutathione levels in HuH-7 cells undergoing oxidative stress (in the presence of BST and HO-1 inh; p < 0.05). Furthermore, Nrf-2 expression and HO-1 gene were significantly enhanced and upregulated respectively in HuH-7 cells pre-treated with RRBE (p < 0.05). Conversely, these effects were attenuated in the presence of brusatol. Conclusion: Antioxidant property of RRBE is mediated through the activation of Nrf-2 and HO-1 pathways. These insights pave way for the development of functional foods or supplementary medicines aimed at preventing and treating NCDs in the future.